Developmental Profile, Morphology, and Synaptic Connectivity of Cajal-Retzius Cells in the Postnatal Mouse Hippocampus.
Developmental Profile, Morphology, and Synaptic Connectivity of Cajal-Retzius Cells in the Postnatal Mouse Hippocampus.
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DOI：
10.1093/cercor/bhv271
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
Lübke JH
中科院分区：
文献类型：
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作者：
Anstötz M;Huang H;Marchionni I;Haumann I;Maccaferri G;Lübke JH
Cajal-Retzius（CR）细胞是早期产生的神经元，参与发育中的新皮层和海马回路的组装。然而，它们在出生后大脑网络中的作用仍然知之甚少。为了深入了解后者的功能，我们研究了它们在CXCR 4-EGFP小鼠出生后海马中的形态学和突触特性，其中CR细胞很容易识别。我们的数据表明，CR细胞是不均匀分布的沿着不同的子领域的海马结构，其出生后的下降是在特定区域的方式进行调节。事实上，CR细胞存在于完全成熟动物的不同区域。CR细胞的亚类投射并靶向海马结构的局部（分子层）或远端区域[下托复合体和内嗅皮层（EC）]，但具有相似的放电模式。最后，CR细胞偏向于靶向树突轴相比，棘，并产生大幅度的γ-氨基丁酸（GABA）的中间神经元上的突触后电位。两者合计，我们的研究结果表明，CR细胞参与了一种新的兴奋性回路的出生后海马结构，这可能有助于塑造海马，海马旁区和内嗅皮层之间的信息流，以及这些脑区的低癫痫发作阈值。
Cajal–Retzius (CR) cells are early generated neurons, involved in the assembly of developing neocortical and hippocampal circuits. However, their roles in networks of the postnatal brain remain poorly understood. In order to get insights into these latter functions, we have studied their morphological and synaptic properties in the postnatal hippocampus of the CXCR4-EGFP mouse, where CR cells are easily identifiable. Our data indicate that CR cells are nonuniformly distributed along different subfields of the hippocampal formation, and that their postnatal decline is regulated in a region-specific manner. In fact, CR cells persist in distinct areas of fully mature animals. Subclasses of CR cells project and target either local (molecular layers) or distant regions [subicular complex and entorhinal cortex (EC)] of the hippocampal formation, but have similar firing patterns. Lastly, CR cells are biased toward targeting dendritic shafts compared with spines, and produce large-amplitude glutamatergic unitary postsynaptic potentials on γ-aminobutyric acid (GABA) containing interneurons. Taken together, our results suggest that CR cells are involved in a novel excitatory loop of the postnatal hippocampal formation, which potentially contributes to shaping the flow of information between the hippocampus, parahippocampal regions and entorhinal cortex, and to the low seizure threshold of these brain areas.