Automated segmentation of electron tomograms for a quantitative description of actin filament networks
Automated segmentation of electron tomograms for a quantitative description of actin filament networks
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DOI：
10.1016/j.jsb.2011.08.012
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发表时间：
2012-01-01
影响因子：
3
通讯作者：
Hege, Hans-Christian
中科院分区：
文献类型：
--
作者：
Rigort, Alexander;Guenther, David;Hege, Hans-Christian
冷冻电子断层扫描允许可视化单个肌动蛋白丝，并描述在未受干扰的细胞环境中肌动蛋白网络的三维组织。对于肌动蛋白丝网络的定量表征，断层图像必须以可再现的方式分割。在这里，我们描述了一个自动化的程序肌动蛋白丝的分割，它结合了模板匹配与一个新的跟踪算法。结果是一组线，每一条线代表灯丝的中心线。正如细胞肌动蛋白网络的冷冻断层图像所示，这些线集可以用于表征细丝网络的细丝长度，方向，密度，刚度（持久性长度）或分支点的出现。(C)2011 Elsevier Inc. All rights reserved.
Cryo-electron tomography allows to visualize individual actin filaments and to describe the three-dimensional organization of actin networks in the context of unperturbed cellular environments. For a quantitative characterization of actin filament networks, the tomograms must be segmented in a reproducible manner. Here, we describe an automated procedure for the segmentation of actin filaments, which combines template matching with a new tracing algorithm. The result is a set of lines, each one representing the central line of a filament. As demonstrated with cryo-tomograms of cellular actin networks, these line sets can be used to characterize filament networks in terms of filament length, orientation, density, stiffness (persistence length), or the occurrence of branching points. (C) 2011 Elsevier Inc. All rights reserved.