Quantitative Gd-DOTA uptake from cerebrospinal fluid into rat brain using 3D VFA-SPGR at 9.4T
Quantitative Gd-DOTA uptake from cerebrospinal fluid into rat brain using 3D VFA-SPGR at 9.4T
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DOI：
10.1002/mrm.26779
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发表时间：
2018-03-01
影响因子：
3.3
通讯作者：
Benveniste, Helene
中科院分区：
文献类型：
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作者：
Lee, Hedok;Mortensen, Kristian;Benveniste, Helene
目的我们提出了一种基于动态增强磁共振成像(DCE-MRI)的定量技术来评估脑实质中的溶质摄取。方法将小分子顺磁性造影剂(Gd-DOTA)注入脑脊液(CSF)，得到全脑Gd浓度图。方法采用三维可变翻转角度破坏梯度回波(VFA-SPGR)纵向弛豫时间(T1)技术，利用体模的反转恢复松弛增强快速采集(IR-REARE)技术对其准确性进行交叉验证。正常Wistar大鼠经枕大池向脑脊液注入Gd-DOTA，用T1加权像连续扫描约130min。计算动态Gd-DOTA浓度图并估计实质摄取。结果在体模研究中，经发射线圈不均匀校正后，VFA-SPGR序列与IR-REARE序列之间的T1差异约为6%。在活体研究中，对比传输轮廓显示最大实质保留量约为进入大脑池总量的19%。结论建立了9.4T时精确三维对比浓度图的成像策略，并绘制了全脑动态浓度图，以研究溶质通过淋巴系统的传输。新开发的方法将使未来能够对健康和疾病状态下的淋巴系统进行定量研究。Magn Reson Med 79：1568-1578,2018。(C)2017国际医学磁共振学会。
PurposeWe propose a quantitative technique to assess solute uptake into the brain parenchyma based on dynamic contrast-enhanced MRI (DCE-MRI). With this approach, a small molecular weight paramagnetic contrast agent (Gd-DOTA) is infused in the cerebral spinal fluid (CSF) and whole brain gadolinium concentration maps are derived.MethodsWe implemented a 3D variable flip angle spoiled gradient echo (VFA-SPGR) longitudinal relaxation time (T1) technique, the accuracy of which was cross-validated by way of inversion recovery rapid acquisition with relaxation enhancement (IR-RARE) using phantoms. Normal Wistar rats underwent Gd-DOTA infusion into CSF via the cisterna magna and continuous MRI for approximately 130min using T1-weighted imaging. Dynamic Gd-DOTA concentration maps were calculated and parenchymal uptake was estimated.ResultsIn the phantom study, T1 discrepancies between the VFA-SPGR and IR-RARE sequences were approximately 6% with a transmit coil inhomogeneity correction. In the in vivo study, contrast transport profiles indicated maximal parenchymal retention of approximately 19% relative to the total amount delivered into the cisterna magna.ConclusionImaging strategies for accurate 3D contrast concentration mapping at 9.4T were developed and whole brain dynamic concentration maps were derived to study solute transport via the glymphatic system. The newly developed approach will enable future quantitative studies of the glymphatic system in health and disease states. Magn Reson Med 79:1568-1578, 2018. (c) 2017 International Society for Magnetic Resonance in Medicine.