Behavioral rhythmicity, age, division of labor and period expression in the honey bee brain
Behavioral rhythmicity, age, division of labor and period expression in the honey bee brain
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DOI：
10.1177/074873001129002123
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发表时间：
2001-10-01
影响因子：
3.5
通讯作者：
Robinson, GE
中科院分区：
文献类型：
--
作者：
Bloch, G;Toma, DP;Robinson, GE
年轻的成年蜜蜂在蜂箱内昼夜不停地“哺育”幼蜂，没有昼夜节律;年长的蜜蜂在外面以强烈的昼夜节律觅食花蜜和花粉。先前的研究表明，在实验室中，在恒定的环境中也可以看到内源性活动节律的发展。新出现的蜜蜂保持在隔离通常是在成年生活的头几天，并发展强大的昼夜节律约几天的年龄。此外，觅食蜂或觅食年龄蜂（> 21日龄）大脑中周期（per）mRNA的平均每日水平高于幼巢蜂（类似于7日龄）。作者使用社会操纵来分离行为节律性、年龄和任务，以确定这些因素与个人行为之间的关系。每个大脑mRNA的平均每日水平与行为节律性或年龄之间没有必然的联系。在促进早熟或逆转行为发育的社会环境中，护士蜜蜂和觅食者之间的每个大脑mRNA水平也没有差异。护士和其他蜂巢年龄的蜜蜂在大脑中的每种mRNA水平可能很高或很低，这取决于社会环境，而觅食者和觅食年龄的蜜蜂总是有很高的水平。这些发现表明蜜蜂觅食行为和per上调之间的联系。结果还表明，任务相关的差异，在每mRNA的振幅振荡在大脑中，觅食者有较大的昼夜波动比护士，无论年龄。综上所述，这些结果表明，社会因素可能会对生物钟基因的调节产生强大的影响。
Young adult honey bees work inside the beehive "nursing" brood around the clock with no circadian rhythms; older bees forage for nectar and pollen outside with strong circadian rhythms. Previous research has shown that the development of an endogenous rhythm of activity is also seen in the laboratory in a constant environment. Newly emerging bees maintained in isolation are typically arrhythmic during the first few days of adult life and develop strong circadian rhythms by about a few days of age. In addition, average daily levels of period (per) mRNA in the brain are higher in foragers or forager-age bees (> 21 days of age) relative to young nest bees (similar to 7 days of age). The authors used social manipulations to uncouple behavioral rhythmicity, age, and task to determine the relationship between these factors and per. There was no obligate link between average daily levels of per brain mRNA and either behavioral rhythmicity or age. There also were no differences in per brain mRNA levels between nurse bees and foragers in social environments that promote precocious or reversed behavioral development. Nurses and other hive-age bees can have high or low levels of per mRNA levels in the brain, depending on the social environment, while foragers and foraging-age bees always have high levels. These findings suggest a link between honey bee foraging behavior and per up-regulation. Results also suggest task-related differences in the amplitude of per mRNA oscillation in the brain, with foragers having larger diurnal fluctuation in per than nurses, regardless of age. Taken together, these results suggest that social factors may exert potent influences on the regulation of clock genes.