Involvement of cyclooxygenase-2 in serum-induced prostaglandin production by human oral gingival epithelial cells
Involvement of cyclooxygenase-2 in serum-induced prostaglandin production by human oral gingival epithelial cells
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DOI：
10.1034/j.1600-0765.2001.360209.x
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发表时间：
2001-04-01
影响因子：
3.5
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
--
作者：
Noguchi, K;Shitashige, M;Ishikawa, I
本研究的目的是探讨环氧合酶-1 (COX-1) 和环氧合酶-2 (COX-2) 在人口腔牙龈上皮 (OGE) 细胞产生前列腺素 (PG) 过程中的参与情况，其中促炎细胞因子包括白细胞介素 (IL)-1 α、IL-1 β 和肿瘤坏死因子 α (TNF α) 以及血清刺激。胎牛血清(FBS)刺激的OGE细胞产生显着水平的PGE-1，而IL-1α、IL-1β和TNFx不能诱导显着水平的PGE(2)产生。 FBS 以剂量和时间依赖性方式诱导 PGE(2) 产生。 NS-398 是一种选择性 COX-2 抑制剂，与非选择性 COX-1/COX-2 抑制剂吲哚美辛一样完全抑制 FBS 刺激的细胞产生 PGE(2)。与未刺激的细胞相比，FBS刺激的细胞中COX-2蛋白的表达增加，而COX-1蛋白P的表达在未刺激的细胞和FBS刺激的细胞中相似。 COX-2 mRNA 在 FBS 刺激的细胞中检测到，但在未刺激的细胞中未检测到。我们认为 COX-2 负责血清刺激的人 OGE 细胞产生 PG，并且 OGE 细胞可能参与牙周病变中 PG 的产生。选择性COX-2抑制剂具有降低胃毒性的优点，可能为牙周病的治疗提供一种有用的方法。
The purpose of the present study was to investigate the involvement of cyclooxygense-1(COX-1) and cyclooxygenase-2 (COX-2) in prostaglandin ( PG) production by human oral gingival epithelial (OGE) cells stimulated with proinflammatory cytokines including interleukin(IL)-1 alpha, IL-1 beta and tumor necrosis factor alpha (TNF alpha), and serum. Fetal bovine serum (FBS)-stimulated OGE cells produced significant levels of PGE-I whereas IL-1 alpha, IL-1 beta and TNFx could not induce significant PGE(2) production. FBS induced PGE(2) production in a dose- and time-dependent manner. NS-398, a selective COX-2 inhibitor, inhibited PGE(2) production by FBS-stimulated cells as completely as indomethacin, a non-selective COX-1/COX-2 inhibitor. Expression of COX-2 protein in FBS-stimulated cells was increased, compared with that in unstimulated cells, whereas COX-1 protein P expression v;as similar both in unstimulated and in FBS-stimulated cells. COX-2 mRNA was detected in FBS-stimulated cells, but not in unstimulated cells. We suggest that COX-2 is responsible fur PG production by human OGE cells stimulated with serum and that OGE cells may be involved in PG production in periodontal Lesions. Selective COX-2 inhibitors, which have the advantage of reduced gastric toxicity, may provide a useful approach to treatment of periodontal disease.