DECREASED LEVEL OF CERAMIDES IN STRATUM-CORNEUM OF ATOPIC-DERMATITIS - AN ETIOLOGIC FACTOR IN ATOPIC DRY SKIN
DECREASED LEVEL OF CERAMIDES IN STRATUM-CORNEUM OF ATOPIC-DERMATITIS - AN ETIOLOGIC FACTOR IN ATOPIC DRY SKIN
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DOI：
10.1111/1523-1747.ep12470233
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发表时间：
1991-04-01
影响因子：
6.5
通讯作者：
HIDANO, A
中科院分区：
文献类型：
--
作者：
IMOKAWA, G;ABE, A;HIDANO, A
角质层脂质是角质层中保水功能和渗透屏障功能的重要决定因素。 然而，它们的主要成分神经酰胺在皮肤病如特应性皮炎中没有被详细分析，这些皮肤病显示出有缺陷的保水性和渗透屏障功能。 为了评估特应性皮炎中每单位质量角质层中神经酰胺的量，通过用氰基丙烯酸酯树脂剥离从前臂皮肤去除角质层片，并置于己烷/乙醇提取中以产生角质层脂质。 通过用二甲基甲酰胺溶解氰基丙烯酸酯树脂来分散角质层，并且在膜过滤之后，测量角质层块的重量。 通过薄层色谱法定量神经酰胺，并以μ g/mg角质层进行评价。 在前臂皮肤的健康人（n = 65），总神经酰胺含量显着下降，随着年龄的增长。 在特应性皮炎（n = 32-35）中，与同龄健康个体相比，病变前臂皮肤中神经酰胺的量显著减少。 有趣的是，非病变皮肤也表现出类似的神经酰胺显著减少。 在六种神经酰胺组分中，神经酰胺1在病变和非病变皮肤中均最显著降低。 这些发现表明，角质层中神经酰胺的不足是特应性干燥皮肤的病因。
Stratum corneum lipids are an important determinant for both water-retention function and permeability-barrier function in the stratum corneum. However, their major constituent, ceramides, have not been analyzed in detail in skin diseases such as atopic dermatitis that show defective water-retention and permeability-barrier function. In an attempt to assess the quantity of ceramides per unit mass of the stratum corneum in atopic dermatitis, stratum corneum sheet was removed from the forearm skin by stripping with cyanoacrylate resin and placed in hexane/ethanol extraction to yield stratum corneum lipids. The stratum corneum was dispersed by solubilization of cyanoacrylate resin with dimethylformamide, and after membrane filtration, the weight of the stratum corneum mass was measured. The ceramides were quantified by thin-layer chromatography and evaluated as mu-g/mg stratum corneum. In the forearm skin of healthy individuals (n = 65), the total ceramide content significantly declined with increasing age. In atopic dermatitis (n = 32-35), there was a marked reduction in the amount of ceramides in the lesional forearm skin compared with those of healthy individuals of the same age. Interestingly, the non-lesional skin also exhibited a similar and significant decrease of ceramides. Among six ceramide fractions, ceramide 1 was most significantly reduced in both lesional and non-lesional skin. These findings suggest that an insufficiency of ceramides in the stratum corneum is an etiologic factor in atopic dry skin.