Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells
Magnolol enhances adipocyte differentiation and glucose uptake in 3T3-L1 cells
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DOI：
10.1016/j.lfs.2009.04.001
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发表时间：
2009-06-19
期刊：
影响因子：
6.1
通讯作者：
Woo, Je-Tae
中科院分区：
文献类型：
--
作者：
Choi, Sun-Sil;Cha, Byung-Yoon;Woo, Je-Tae
目的：核受体过氧化物酶体增殖物激活受体（过氧化物酶体增殖物激活受体）γ在脂肪细胞分化中起重要作用。其配体包括噻唑烷二酮类，可改善2型糖尿病的胰岛素敏感性。本研究采用3 T3-L1细胞和C3 H10 T1/2细胞为实验材料，采用Oil Rd 0染色法检测厚朴酚对脂肪细胞分化的影响。采用实时荧光定量PCR和Western blot检测细胞中PPAR γ及其靶基因的表达。分别厚朴酚对3 T3-L1脂肪细胞葡萄糖摄取的影响进行了研究。关键发现：厚朴酚剂量依赖性地促进3 T3-L1细胞和C3 H10 T1/2细胞的脂肪细胞分化。在脂肪形成的早期阶段，厚朴酚诱导C/EBP δ、C/EBP α和PPARgamma 2的基因表达，在脂肪细胞分化过程中，厚朴酚还诱导PPARgamma靶基因如aP 2、LPL和脂联素的表达。此外，厚朴酚还在mRNA和aP 2蛋白水平上增加成熟脂肪细胞中PAPR γ靶基因如C/EBP α和aP 2的表达。在PPAR γ配体结合试验中，厚朴酚表现出与PPAR γ的结合亲和力，但其活性弱于罗格列酮。同时，GW 9662对313-L1细胞和C3 H10 T1/2细胞的成脂作用也有抑制作用。在成熟的3 T-3-L1脂肪细胞中，厚朴酚可增加基础和胰岛素刺激的葡萄糖摄取，并上调Glut 4的mRNA和蛋白水平。意义：厚朴酚可能通过激活PPAR γ作为配体而提高胰岛素敏感性。(C)2009 Elsevier Inc. All rights reserved.
Aims: The nuclear receptor peroxisome proliferator-activated receptor (PPAR) gamma plays an important role in adipocyte differentiation. its ligands, including thiazolidinediones, improve insulin sensitivity in type 2 diabetes. We investigate the effect of magnolol, an ingredient of Magnolia officinalis on adipogenesis and glucose uptake using 3T3-L1 cells.Main methods: The effect of magnolol on adipocyte differentiation was quantified by measuring Oil Rd 0 staining using 3T3-L1 cells and C3H10T1/2 cells. And real-time PCR and western blot were used to determine the expression of PPAR gamma or PPAR gamma target genes. respectively. The effect of magnolol on glucose uptake was performed using 3T3-L1 adipocytes.Key findings: Magnolol dose-dependently enhanced adipocyte differentiation in 3T3-L1 cells and C3H10T1/2 cells. In the early stage of adipogenesis, magnolol induced gene expression of C/EBP delta, C/EBP alpha and PPAR gamma 2 and during adipocyte differentiation, it also induced the expression of PPAR gamma target genes such as aP2, LPL and adiponectin. In addition, magnolol it also increase expression of PAPR gamma target gene such as C/EBP alpha and aP2 at mRNA and aP2 protein level in mature adipocytes. In PPAR gamma ligand binding assays, magnolol exhibited binding affinity to PPAR gamma but its activity was weaker than rosiglitazone. At the same time, magnolol-induced adipogenesis was inhibited by co-treatment of GW9662 both 313-L1 cells and C3H10T1/2 cells. In mature 3T-3-L1 adipocytes, magnolol increased basal and insulin-stimulated glucose uptake accompanied by the up-regulation of mRNA and protein level of Glut4.Significance: Our results suggest that magnolol could improve insulin sensitivity through the activation of PPAR gamma as a ligand. (C) 2009 Elsevier Inc. All rights reserved.