MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles
MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles
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DOI：
10.1002/mrm.10406
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发表时间：
2003-03-01
影响因子：
3.3
通讯作者：
Bhujwalla, ZM
中科院分区：
文献类型：
--
作者：
Artemov, D;Mori, N;Bhujwalla, ZM
磁共振分子成像在磁共振生物医学应用中是一个令人兴奋的新前沿领域。临床相关靶点之一是酪氨酸激酶Her - 2/neu受体，它在乳腺癌的分期和治疗中具有重要作用。在这项研究中，对一组在细胞膜上表达不同数量受体的乳腺癌细胞中的Her - 2/neu受体进行了成像。使用市售的链霉亲和素偶联的超顺磁性纳米粒子作为靶向磁共振造影剂。这些纳米粒子被导向预先用生物素化单克隆抗体标记的受体，并在表达Her - 2/neu的细胞中产生强烈的T - 2磁共振对比。在磁共振图像中观察到的对比与通过流式细胞术分析独立确定的Her - 2/neu受体表达水平成正比。在这些实验中，氧化铁纳米粒子附着在细胞表面，没有被细胞内化，这是该方法在体内应用的一个主要优势。（C）2003威利 - 利斯公司
MR molecular imaging is an exciting new frontier in the biomedical applications of MR. One of the clinically relevant targets is the tyrosine kinase Her-2/neu receptor, which has a significant role in staging and treating breast cancer. In this study Her-2/ neu receptors were imaged in a panel of breast cancer cells expressing different numbers of the receptors on the cell membrane. Commercially available streptavidin-conjugated superparamagnetic nanoparticles were used as targeted MR contrast agent. The nanoparticles were directed to receptors prelabeled with a biotinylated monoclonal antibody and generated strong T-2 MR contrast in Her-2/neu-expressing cells. The contrast observed in MR images was proportional to the expression level of Her-2/neu receptors determined independently with FACS analysis. In these experiments, iron oxide nanoparticles were attached to the cell surface and were not internalized into the cells, which is a major advantage for in vivo applications of the method. (C) 2003 Wiley-Liss, Inc.