Nonpathogenic Escherichia coli strain Nissle1917 prevents murine acute and chronic colitis
Nonpathogenic Escherichia coli strain Nissle1917 prevents murine acute and chronic colitis
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DOI：
10.1097/01.mib.0000158158.55955.de
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发表时间：
2005-05-01
影响因子：
4.9
通讯作者：
Hibi, T
中科院分区：
文献类型：
--
作者：
Kamada, A;Inoue, N;Hibi, T
背景资料：非致病性大肠杆菌菌株Nissle 1917已被用作人类炎症性肠病的益生菌;然而，很少有研究其对动物结肠炎模型的治疗效果的报道，其治疗机制仍然未知。本研究采用小鼠急、慢性结肠炎模型，探讨Nissle 1917的治疗作用及其机制。(1)急性模型：采用1.3%葡聚糖硫酸钠灌胃7天诱导大鼠结肠炎模型。口服Nissle 1917或磷酸盐缓冲盐水10天。在第10天处死小鼠，用肉眼和显微镜评估结肠病变。(2)慢性模型：IL-10（-/-）小鼠用Nissle 1917或磷酸盐缓冲盐水处理8周。治疗8周后，处死小鼠，通过肉眼和显微镜观察结肠病变。在急性葡聚糖硫酸钠结肠炎模型中，口服Nissle 1917的活DNA、热灭活DNA或基因组DNA 10天，评价治疗效果。Nissle 1917改善体重减轻、疾病活动指数。以及宏观和微观损伤。在慢性模型中，它还抑制粘膜炎症和组织学损伤。此外，热灭活Nissle 1917或其基因组DNA单独也改善了急性DSS结肠炎和活菌的宏观和微观观察。大肠杆菌菌株Nissle 1917可预防急性和慢性结肠炎，其抗炎作用不仅通过活细菌而且通过热灭活细菌或其DNA表现出来。
Background: Nonpathogenic Escherichia coli strain Nissle1917 has been used as a probiotics in human inflammatory bowel disease; however, there are few reports examining its therapeutic effect on animal colitis models, and its therapeutic mechanisms remain unknown. The aim of this study was to elucidate the therapeutic effect and mechanism of Nissle 1917 using murine acute and chronic colitis models.Methods: Two models were used. (1) Acute model: colitis was induced by administration of 1.3% dextran sodium sulfate for 7 days. Nissle 1917 or phosphate-buffered saline were orally administered for l 0 days. Mice were killed at day 10, and the colonic lesions were assessed macro- and microscopically. (2) Chronic model: IL-10(-/-) mice were treated with Nissle1917 or phosphate-buffered saline for 8 weeks. After 8 weeks of treatment, mice were killed to assess the colonic lesions macro- and microscopically In the acute dextran sodium sulfate colitis model, viable, heat-killed, or genomic DNA of Nissle 1917 was orally administered for 10 days, and the therapeutic effect was assessed.Results: In the acute model. Nissle1917 ameliorated body weight loss, disease activity index.. and macro- and microscopic damage. In the chronic model, it also suppressed the mucosal inflammatory findings and histologic damages. Moreover, heat-killed Nissle 1917 or its genomic DNA alone also ameliorated the acute DSS colitis and viable bacteria macro- and microscopically.Conclusions: Nonpathogenic E. coli strain Nissle1917 prevents both acute and chronic colitis, and its anti-inflammatory effect is exhibited not only by viable bacteria but also by heat-killed bacteria or its DNA.