Visualization and experimental analysis of blood vessel formation using Transgenic zebrafish
Visualization and experimental analysis of blood vessel formation using Transgenic zebrafish
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DOI：
10.1002/bdrc.20103
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发表时间：
2007-12-01
影响因子：
2.1
通讯作者：
Weinstein, Brant M.
中科院分区：
文献类型：
--
作者：
Cha, Young Ryun;Weinstein, Brant M.
血管形成的机制已经成为具有巨大科学和临床意义的课题。然而，由于血管在其他组织中无处不在和深度定位，因此难以可视化大多数活体动物中发育的脉管系统。血管特异性转基因斑马鱼荧光“标记”血管的建立，促进了高分辨率成像研究发展中的血液和淋巴管在体内。使用这些转基因系进行遗传和化学筛选、实验操作和延时成像扩展了我们对复杂血管网络如何在体内组装的了解。
The mechanisms of blood vessel formation have become a subject of enormous scientific and clinical interest. However, it is difficult to visualize the developing vasculature in most living animals due to the ubiquitous and deep localization of vessels within other tissues. The establishment of vascular-specific transgenic zebrafish with fluorescently "tagged" blood vessels has facilitated high-resolution imaging studies of developing blood and lymphatic vessels in vivo. Use of these transgenic lines for genetic and chemical screening, experimental manipulations, and time-lapse imaging has extended our knowledge of how complex networks of vessels assemble in vivo.