Citrulluside H and citrulluside T from young watermelone (Citrullus lanatus) fruit attenuate ultraviolet B radiation-induced matrix metalloproteinase expression through the scavenging of generated reactive oxygen species in human dermal fibroblast.
Citrulluside H and citrulluside T from young watermelone (Citrullus lanatus) fruit attenuate ultraviolet B radiation-induced matrix metalloproteinase expression through the scavenging of generated reactive oxygen species in human dermal fibroblast.
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来自年轻西瓜 (Citrullus lanatus) 果实的西瓜苷 H 和西瓜苷 T 通过清除人真皮成纤维细胞中产生的活性氧来减弱紫外线 B 辐射诱导的基质金属蛋白酶的表达。
DOI：
10.1111/phpp.12669
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Toshiharu Hashizume.
中科院分区：
文献类型：
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作者：
Tomohiro Itoh;Singo Fujita;Masayuki NInomiya;Mamoru Koketsu;Toshiharu Hashizume.
长期暴露在紫外线辐射下会破坏氧化应激防御系统；在缺乏足够的活性氧（ROS）清除机制的情况下，产生的ROS会对DNA、蛋白质、碳水化合物和脂质造成氧化损伤。因此，消除活性氧可以防止光老化和可能的皮肤癌。从西瓜幼果中分离到两种具有抗氧化活性的新型酚类苷（瓜氨酸苷H和瓜氨酸苷T）。在本研究中，我们评估了这些化合物对UV - b诱导的正常人真皮成纤维细胞（NHDFs）光损伤的保护作用。方法将snhdfs暴露于25 mJ/cm2的UV - B辐射下。采用实时荧光定量PCR检测基质金属蛋白酶（MMP）‐1和MMP‐3 mRNA水平。Western blot分析MMP蛋白表达、MAPK和NF - κB信号传导。激活蛋白- 1 （AP - 1）转录活性采用荧光素酶报告基因法测定。使用CellROX™Deep Red Reagent检测细胞内ROS水平。结果瓜鲁luside H和瓜鲁luside T显著下调与皮肤皱纹形成有关的MMP‐1和MMP‐3蛋白和mRNA的表达。重要的是，瓜氨酸苷H和瓜氨酸苷T对UV - b诱导的NHDFs中MMP表达的抑制主要是由于通过清除产生的ROS来减弱MAPK/AP - 1和NF - κB信号。结论：我们的研究结果表明瓜氨酸H和瓜氨酸T都是缓解紫外线诱导的皮肤光老化的潜在药物。
BackgroundProlonged exposure to UV radiation disrupts the oxidative stress defense systems; in the absence of adequate reactive oxygen species (ROS)‐scavenging mechanisms, the ROS produced cause oxidative damage to DNA, proteins, carbohydrates, and lipids. Thus, ROS elimination can protect against photoaging and possibly skin cancer. We recently isolated two novel phenolic glycosides (citrulluside H and citrulluside T) with antioxidative activity from young watermelon (Citrullus lanatus) fruits. In the present study, we evaluated the protective effect of these compounds against UV‐B–induced photodamage in normal human dermal fibroblasts (NHDFs).MethodsNHDFs were exposed to UV‐B irradiation at 25 mJ/cm2. Matrix metalloproteinase (MMP)‐1 and MMP‐3 mRNA levels were measured by quantitative real‐time PCR. MMP protein expression, as well as MAPK and NF‐κB signaling, was investigated by Western blot analysis. Activation protein‐1 (AP‐1) transcriptional activity was determined using a luciferase reporter assay. Intracellular ROS levels were measured using the CellROX™ Deep Red Reagent.ResultsCitrulluside H and citrulluside T significantly downregulated the protein and mRNA expression of MMP‐1 and MMP‐3, which are associated with the onset of skin wrinkle formation. Importantly, inhibition of UV‐B–induced MMP expression in NHDFs by citrulluside H and citrulluside T was primarily due to the attenuation of MAPK/AP‐1 and NF‐κB signaling via scavenging of the generated ROS.ConclusionOur findings delineated both citrulluside H and citrulluside T as potential agents for alleviating UV‐induced skin photoaging.