Localization of neuropeptide Y messenger ribonucleic acid in rat and mouse brian by in situ hybridization-猫眼课题宝

通过原位杂交对大鼠和小鼠脑中神经肽 Y 信使核糖核酸进行定位

DOI：

发表时间：

1987

期刊：

Synapse

影响因子：

2.3

通讯作者：

中科院分区：

医学4区

文献类型：

作者：

D. Gehlert;B. Chronwall;M. Schafer;T. O'Donohue

本文研究了神经肽Y（NPY）信使核糖核酸（mRNA）在大、小鼠脑内的分布。通过使用末端转移酶，用[35 S]-脱氧胞嘧啶三磷酸（[35 S]-dCTP）标记与NPY mRNA的独特区域互补的28-mer寡核苷酸探针的3′-末端，并与大鼠和小鼠脑的固定切片杂交。然后放射自显影检测标记探针与组织切片中mRNA的杂交。在下丘脑弓状核区观察到较强的杂交信号。在整个大脑皮质中观察到散在的含NPY-mRNA的细胞体。实验比较了NPY mRNA的分布与化学检测的NPY，表明在下丘脑弓状核和大脑皮层中有相似的分布。这些结果表明，NPY基因的主动转录发生在脑内，并且这种转录发生在含有NPY的细胞体中。

The distribution of neuropeptide Y (NPY) messenger ribonucleic acid (mRNA) in the rat and mouse brain has been examined. A 28‐mer oligonucleotide probe complementary to a distinctive region of NPY mRNA was 3′‐end labeled with [35S]‐deoxycytosine triphosphate ([35S]‐dCTP) by using terminal transferase and was hybridized to fixed sections of rat and mouse brain. The hybridization of the labeled probe to the mRNA in the tissue section was then detected autoradiographically. A strong hybridization signal was seen corresponding to the arcuate nucleus of the hypothalamus. Scattered NPY‐mRNA‐containing cell bodies were seen throughout the cerebral cortex. Experiments which compared the distribution of NPY mRNA with immunohistochemically detected NPY indicated a similar distribution in the arcuate nucleus of the hypothalamus and cerebral cortex. These results indicate that active transcription of the NPY gene occurs within the brain and this transcription takes place in NPY‐containing cell bodies.