Dietary patterns reflecting healthy food choices are associated with lower serum LPS activity
Dietary patterns reflecting healthy food choices are associated with lower serum LPS activity
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DOI：
10.1038/s41598-017-06885-7
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发表时间：
2017-07-26
影响因子：
4.6
通讯作者：
Groop, Per-Henrik
中科院分区：
文献类型：
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作者：
Ahola, Aila J.;Lassenius, Mariann I.;Groop, Per-Henrik
革兰氏阴性菌衍生的脂多糖 (LPS) 与各种负面健康影响有关。饮食是否与脂多糖有关，目前尚未得到充分研究。我们在 FinnDiane 研究中调查了 668 名 1 型糖尿病患者的饮食与血清 LPS 活性之间的关联。使用鲎变形细胞裂解物测定法测定血清LPS活性。通过饮食调查问卷的食物频率调查问卷（FFQ）部分和食物记录来评估饮食。食物记录用于计算能量、常量营养素和纤维摄入量。在多变量模型中，能量、常量营养素或纤维摄入量与 LPS 活性无关。通过因子分析，我们从 FFQ 数据中确定了七种饮食模式（“甜”、“奶酪”、“鱼”、“健康零食”、“蔬菜”、“传统”和“现代”）。在多变量模型中，鱼类、健康零食和现代模式的因子得分越高，则预测 LPS 活性越低。饮食问卷的有效性也进行了调查。调查问卷显示出针对 6 天饮食记录的合理相对有效性。这两种方法将参与者分类的饮食模式比偶然预期的要好。总之，健康的饮食选择，例如食用鱼类、新鲜蔬菜、水果和浆果，可能通过减少全身性内毒素血症而与积极的健康结果相关。
Gram-negative bacteria-derived lipopolysaccharides (LPS) are associated with various negative health effects. Whether diet is associated with LPS, is an understudied phenomenon. We investigated the association between diet and serum LPS activity in 668 individuals with type 1 diabetes in the FinnDiane Study. Serum LPS activity was determined using the Limulus Amoebocyte Lysate assay. Diet was assessed with a food frequency questionnaire (FFQ) section of a diet questionnaire and a food record. The food record was used to calculate energy, macronutrient, and fibre intake. In a multivariable model, energy, macronutrient, or fibre intake was not associated with the LPS activity. Using factor analysis, we identified seven dietary patterns from the FFQ data ("Sweet", "Cheese", "Fish", "Healthy snack", "Vegetable", "Traditional", and "Modern"). In a multivariable model, higher factor scores of the Fish, Healthy snack, and Modern patterns predicted lower LPS activity. The validity of the diet questionnaire was also investigated. The questionnaire showed reasonable relative validity against a 6-day food record. The two methods classified participants into the dietary patterns better than expected by chance. In conclusion, healthy dietary choices, such as consumption of fish, fresh vegetables, and fruits and berries may be associated with positive health outcomes by reducing systemic endotoxaemia.