An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2af1-rs1 gene marks the establishment of allele-specific methylation during preimplantation development.
An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2af1-rs1 gene marks the establishment of allele-specific methylation during preimplantation development.
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小鼠 U2afbp-rs/U2af1-rs1 基因中的卵母细胞特异性甲基化印记中心标志着植入前发育过程中等位基因特异性甲基化的建立。
DOI：
10.1006/geno.1997.4877
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Hayashizaki,Y
中科院分区：
文献类型：
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作者：
Shibata,H;Ueda,T;Kamiya,M;Yoshiki,A;Kusakabe,M;Plass,C;Held,WA;Sunahara,S;Katsuki,M;Muramatsu,M;Hayashizaki,Y
在小鼠印迹基因U2 afbp-rs的约200 bp处，发现了一个卵母细胞特异性甲基化印迹标记区域，该区域包含一个CpG岛和一个短串联重复序列。卵母细胞特异性甲基化保留在受精卵中，然后在植入前发育过程中在被抑制的母体等位基因上扩增，直到胚胎发育12.5天达到成年甲基化模式。这些结果表明，卵母细胞特异性印迹标记区域作为一个中心，在建立高甲基化区域的抑制母亲的等位基因。此外，在两个配子中均发现了一个高甲基化区域，但其高甲基化仅在植入前发育期间在母体等位基因上保守，这表明从卵母细胞遗传的一些因子可能起作用以维持母体等位基因的高甲基化。
An oocyte-specific methylation imprint mark region, consisting of approximately 200 bp from the mouse imprinted geneU2afbp-rs,was identified within an area containing a CpG island and a short tandem repeat sequence. The oocyte-specific methylation was preserved in fertilized eggs and then expanded on the repressed maternal allele during preimplantation development until the adult methylation pattern was achieved by 12.5 days of embryonic development. These results indicate that the oocyte-specific imprinting mark region acts as a center in establishing the hypermethylated region on the repressed maternal allele. Furthermore, a region that is hypermethylated in both gametes was identified but its hypermethylation was conserved only on the maternal allele during preimplantation development, suggesting that some factor(s) inherited from oocytes may act to maintain hypermethylation on the maternal allele.