Hsp90 Regulates Nongenetic Variation in Response to Environmental Stress
Hsp90 Regulates Nongenetic Variation in Response to Environmental Stress
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DOI：
10.1016/j.molcel.2013.01.026
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发表时间：
2013-04-11
期刊：
影响因子：
16
通讯作者：
Leu, Jun-Yi
中科院分区：
文献类型：
--
作者：
Hsieh, Yu-Ying;Hung, Po-Hsiang;Leu, Jun-Yi
细胞间的非遗传变异往往对克隆群体在波动环境中的生存起着重要作用。然而，在大多数生物体中，调节这种非遗传异质性的潜在机制仍然难以捉摸。我们在这里报道，克隆酵母群体表现出形态异质性当Hsp90的水平，分子伴侣，降低。这种形态异质性是由Cdc28和cl4的剂量驱动的，cl4是septin形成的关键调节因子。低Hsp90水平降低了cl4蛋白的稳定性，并导致细胞亚群转变为丝状形式，这在之前被认为在某些恶劣环境下是有益的。此外，hsp90依赖的形态异质性可由环境胁迫诱导，并在不同酵母物种中保守。我们的研究结果表明，Hsp90提供了一种进化上保守的机制，将环境胁迫与诱导形态多样性联系起来。
Nongenetic cell-to-cell variability often plays an important role for the survival of a clonal population in the face of fluctuating environments. However, the underlying mechanisms regulating such nongenetic heterogeneity remain elusive in most organisms. We report here that a clonal yeast population exhibits morphological heterogeneity when the level of Hsp90, a molecular chaperone, is reduced. The morphological heterogeneity is driven by the dosage of Cdc28 and Cla4, a key regulator of septin formation. Low Hsp90 levels reduce Cla4 protein stability and cause a subpopulation of cells to switch to a filamentous form that has been previously suggested to be beneficial under certain hostile environments. Moreover, Hsp90-dependent morphological heterogeneity can be induced by environmental stress and is conserved across diverse yeast species. Our results suggest that Hsp90 provides an evolutionarily conserved mechanism that links environmental stress to the induction of morphological diversity.