Molecular Survey and Phylogenetic Analysis of Atypical Porcine Pestivirus (APPV) Identified in Swine and Wild Boar from Northern Italy
Molecular Survey and Phylogenetic Analysis of Atypical Porcine Pestivirus (APPV) Identified in Swine and Wild Boar from Northern Italy
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DOI：
10.3390/v11121142
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发表时间：
2019-12-01
期刊：
影响因子：
4.7
通讯作者：
Lavazza, Antonio
中科院分区：
文献类型：
--
作者：
Sozzi, Enrica;Salogni, Cristian;Lavazza, Antonio
非典型猪瘟病毒（APPV）是一种新发现的黄病毒科成员。这种新型猪瘟病毒于2015年在美国首次被描述，它与新生仔猪的先天性震颤A-II型有关。APPV在欧洲和亚洲的家猪中广泛分布。在这项研究中，在北方意大利进行了病毒学调查，以调查存在的APPV分子方法。对来自猪群的360个流产样本进行的测试显示，两种APPV毒株来自伦巴第地区不同省份，对430个野猪血液样本进行的测试显示，三种毒株，一种来自伦巴第，两种来自艾米利亚罗马涅。非结构蛋白3编码区的一个片段的核苷酸序列分析显示，与先前检测到的欧洲株（西班牙，德国和意大利）的APPV高度相似。
Atypical porcine pestivirus (APPV) is a newly recognized member of the Flaviviridae family. This novel porcine pestivirus was first described in 2015 in the USA, where it has been associated with congenital tremor type A-II in new-born piglets. APPV is widely distributed in domestic pigs in Europe and Asia. In this study, a virological survey was performed in Northern Italy to investigate the presence of APPV using molecular methods. Testing of 360 abortion samples from pig herds revealed two APPV strains from distinct provinces in the Lombardy region and testing of 430 wild boar blood samples revealed three strains, one from Lombardy and two from Emilia Romagna. The nucleotide sequencing of a fragment of the nonstructural protein 3-coding region revealed a high similarity to the previously detected European strains (Spanish, German, and Italian) of APPV.