Time course variations of antioxidant enzyme activities and histopathology of gilthead seabream gills exposed to malathion.
Time course variations of antioxidant enzyme activities and histopathology of gilthead seabream gills exposed to malathion.
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DOI：
10.14670/hh-20.1017
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发表时间：
2005-10
影响因子：
2
通讯作者：
M. Rosety;M. Rosety-Rodriguez;F. J. Ordoñez;I. Rosety
中科院分区：
文献类型：
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作者：
M. Rosety;M. Rosety-Rodriguez;F. J. Ordoñez;I. Rosety
在一种广泛分布和商业上重要的鱼类，金头鲷鲷，我们研究了马拉硫磷的亚致死效应，以便在不可逆转的损害发生之前确定暴露的预警生物指标。为了实现这一目标，10个少年标本组暴露于24，48，72和96小时的马拉硫磷（0.4毫克/升）的亚致死浓度。另一组作为对照。抗氧化酶（超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶）的活性和暴露的鳃组织病理学特征进行了评估。还应提及的是，在整个实验过程中未观察到死亡。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）活性从24 h开始显著变化（p<0.05）。值得注意的是，过氧化氢酶活性在暴露后降低，而不是像其他抗氧化酶那样升高。另一方面，组织病理学改变的鳃观察早在48小时曝光，但最严重的损害发生在96小时曝光。这里提出的证据，连同其他数据从文献中，明确建立了马拉硫磷的氧化应激诱导作用的金头鲷。此外，在鳃的组织病理学变化明显之前，抗氧化剂（SOD、CAT和GPX）的变化很长一段时间。因此，这些抗氧化酶可作为马拉硫磷环境污染的早期预警生物指示剂，在建议用于害虫控制活动的地区。
In a widely distributed and commercially important fish, gilthead seabream Sparus aurata L., we have studied sublethal effects of malathion in order to identify early warning bioindicators of exposure before irreversible damage occurs. To achieve this goal, groups of 10 juvenile specimens were exposed for 24, 48, 72 and 96 h to a sublethal concentration of malathion (0.4 mg/l). Another group was used as control. The activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) and histopathological features from exposed gills were assessed. It should also be mentioned that no mortality was observed during the whole experience. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were altered significantly from 24 h onward (p<0.05). It is of interest to note that catalase activity was decreased after exposure instead of increasing as other antioxidant enzymes assessed. On the other hand, histopathological alterations of the gills were observed as early as at 48 h-exposure, but the most severe damage occurred at 96 h exposure. The evidence presented here, together with other data from the literature, unequivocally established oxidative-stress-inducing effects of malathion in gilthead seabream Sparus aurata. It is also concluded antioxidants employed (SOD, CAT and GPX) changed significantly a long time before histopathological alterations of gills became evident. Consequently, these antioxidant enzymes may be highly recommended as early-warning bioindicators of environmental pollution by malathion in the areas where it is proposed to be used in pest control activities.