A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles.
A metabolomic study on the responses of daphnia magna exposed to silver nitrate and coated silver nanoparticles.
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DOI：
10.1016/j.ecoenv.2015.05.005
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发表时间：
2015-09
影响因子：
6.8
通讯作者：
Lianzhen Li;Huifeng Wu;Chenglong Ji;C. V. van Gestel;H. Allen;W. Peijnenburg
中科院分区：
文献类型：
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作者：
Lianzhen Li;Huifeng Wu;Chenglong Ji;C. V. van Gestel;H. Allen;W. Peijnenburg
我们使用基于~1H核磁共振的代谢组学方法，在亚致死水平上检测了AgNPs和AgNO3对大型水蚤的短期毒性。合成了10和40 nm两种粒径的聚乙烯吡咯烷酮包覆银纳米粒子，并对其进行了表征，用离心超滤和电感耦合等离子体质谱研究了它们的Ag+释放。对核磁共振氢谱的多元统计分析表明，核磁共振氢谱有明显的变化。暴露于AgNP颗粒大小和Ag+暴露48小时后的磁代谢分布。大多数AgNP暴露的代谢生物标志物，包括3-羟基丁酸、精氨酸、赖氨酸和磷胆碱，与Ag+暴露组相同，表明两种AgNP的显性作用是由于释放了Ag+。观察到的代谢变化表明，释放的Ag+导致能量代谢紊乱和氧化应激，这是AgNP毒性的一个可能机制。所有AgNP处理组的乳酸水平升高，但Ag+处理组没有，这为Ag-NP增强无氧代谢提供了证据。这些发现表明，基于~1H核磁共振的代谢组学提供了一种检测糖尿病的灵敏方法。此外，还需要进一步的靶向分析，以阐明纳米颗粒诱导毒性的作用机制。
We examined the short-term toxicity of AgNPs and AgNO3to Daphnia magna at sublethal levels using1H NMR-based metabolomics. Two sizes of polyvinylpyrrolidone-coated AgNPs (10 and 40 nm) were synthesized and characterized and their Ag+release was studied using centrifugal ultrafiltration and inductively coupled plasma mass spectrometry. Multivariate statistical analysis of the1H NMR spectra showed significant changes in theD. magnametabolic profiles following 48 h exposure to both AgNP particle sizes and Ag+exposure. Most of the metabolic biomarkers for AgNP exposure, including 3-hydroxybutyrate, arginine, lysine and phosphocholine, were identical to those of the Ag+-exposed groups, suggesting that the dominant effects of both AgNPs were due to released Ag+. The observed metabolic changes implied that the released Ag+induced disturbance in energy metabolism and oxidative stress, a proposed mechanism of AgNP toxicity. Elevated levels of lactate in all AgNP-treated but not in Ag+-treated groups provided evidence for Ag-NP enhanced anaerobic metabolism. These findings show that1H NMR-based metabolomics provides a sensitive measure ofD. magnaresponse to AgNPs and that further targeted assays are needed to elucidate mechanisms of action of nanoparticle-induced toxicity.