Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells
Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells
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DOI：
10.1016/j.febslet.2010.09.047
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发表时间：
2010-11-05
期刊：
影响因子：
3.5
通讯作者：
Tani, Katsuko
中科院分区：
文献类型：
--
作者：
Sato, Sei-ichi;Inoue, Hiroki;Tani, Katsuko
哺乳动物细胞内磷脂酶A(1)蛋白家族有三个成员（磷脂酸偏好-磷脂酶A(1)、p125和KIAA0725p）。在这项研究中，我们发现KIAA0725p定位于高尔基体，并在高尔基体和细胞质之间快速循环。催化活性对于KIAA0725p靶向高尔基膜具有重要意义。RNA干扰实验表明KIAA0725p有助于从高尔基体到质膜的有效膜运输，但不参与brefeldin a诱导的高尔基体到内质网的逆行运输。结构化摘要：MINT-8019765: KIAA0725 （uniprotkb:O94830）和β - cop （uniprotkb:P53618）共定位（MI:0403）通过荧光显微镜（MI: 0416）MINT-8019775: KIAA0725 （uniprotkb:O94830）和GM130 （uniprotkb:Q5PXD5）共定位（MI:0403）通过荧光显微镜（MI: 0416） (C) 2010欧洲生化学会联合会。Elsevier B.V.版权所有。
Mammals have three members of the intracellular phospholipase A(1) protein family (phosphatidic acid preferring-phospholipase A(1), p125, and KIAA0725p). In this study, we showed that KIAA0725p is localized in the Golgi, and is rapidly cycled between the Golgi and cytosol. Catalytic activity is important for targeting of KIAA0725p to Golgi membranes. RNA interference experiments suggested that KIAA0725p contributes to efficient membrane trafficking from the Golgi apparatus to the plasma membrane, but is not involved in brefeldin A-induced Golgi-to-endoplasmic reticulum retrograde transport.Structured summary:MINT-8019765: KIAA0725 (uniprotkb:O94830) and Beta-COP (uniprotkb:P53618) colocalize (MI:0403) by fluorescence microscopy (MI: 0416)MINT-8019775: KIAA0725 (uniprotkb:O94830) and GM130 (uniprotkb:Q5PXD5) colocalize (MI:0403) by fluorescence microscopy (MI: 0416) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.