Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains
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DOI：
10.7554/elife.25114
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发表时间：
2017-03-06
期刊：
影响因子：
7.7
通讯作者：
Zipfel, Cyril
中科院分区：
文献类型：
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作者：
Buecherl, Christoph A.;Jarsch, Iris K.;Zipfel, Cyril
在多细胞生物中，细胞表面受体控制着多种信号通路。在植物中，突出的例子是受体激酶FLS2和BRI1，它们分别激活免疫和类固醇介导的生长。有趣的是，尽管诱导不同的信号输出，这两种受体都使用共同的下游信号成分，这些成分存在于质膜（PM）定位的蛋白质复合物中。因此，一个重要的问题是这些受体复合物如何维持信号特异性。活细胞成像显示FLS2和BRI1形成PM纳米团簇。使用单粒子跟踪，我们可以区分这两个集群种群，我们观察到免疫和生长信号平台之间的时空分离。通过观察不同PM纳米结构域内的FLS2和BRI1，这一发现得到了证实，这些纳米结构域以特定的悔恨蛋白和与细胞骨架的差异共定位为标志。因此，我们的研究结果表明，这些途径之间的信号特异性可以通过FLS2和BRI1及其相关信号成分在专用PM纳米结构域中的空间分离来解释。
Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.