iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage.
iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage.
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DOI：
10.1016/j.foodchem.2017.12.004
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发表时间：
2018-05
期刊：
影响因子：
8.8
通讯作者：
Chong Wang;Jianjun Chu;L. Fu;Yanbo Wang;Feng Zhao;Deqing Zhou
中科院分区：
文献类型：
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作者：
Chong Wang;Jianjun Chu;L. Fu;Yanbo Wang;Feng Zhao;Deqing Zhou
文蛤是一种重要的养殖贝类，具有重要的经济价值和较高的营养价值。由于其易腐败的特性，一般采用冻点贮藏法（CFPS）进行保鲜。本研究应用同量异位素标记技术（iTRAQ），探讨了冷应激条件下梭子蟹冷应激适应的生化机制。共检测到369个蛋白质，其中27个蛋白质为CFPS过程中差异表达蛋白质，主要参与能量代谢、DNA复制和蛋白质合成以及应激反应，其中MAPK是主要途径。进一步的qPCR结果显示H2A和S6K 2 α是关键的转录后调节基因。本研究结果为深入研究低温胁迫下蛤蜊适应的生化机制提供了蛋白质组学信息，为进一步延长蛤蜊的贮藏期提供了理论依据，并有助于阐明其新的耐冷机制。
Razor clam is a major cultivated shellfish of great economic importance and high nutritional value. Due to high corruptible potential, razor clam is generally preserved by controlled freezing-point storage (CFPS). Here, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling to investigate the biochemical mechanism of cold stress adaption in razor clam during CFPS. In total, 369 proteins were quantified, and 27 of them were identified as differentially expressed proteins during CFPS, mostly involved in energy metabolism process, DNA duplication and protein synthesis, and stress response, specifically, MAPK is the predominant pathway. Further qPCR results revealed H2A and S6K 2 alpha to be the critical post-transcriptionally regulated genes. Our results provided proteomics information with respect to the biochemical mechanism of cold stress adaption in razor clam, shed light on the further elongation of razor clams storage period, and help clarify the novel mechanisms of cold tolerance.