Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization
Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization
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DOI：
10.1128/jb.184.1.327-330.2002
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Kado, CI
中科院分区：
文献类型：
--
作者：
Lai, EM;Eisenbrandt, R;Kado, CI
VirB2 propilin 在大肠杆菌和根癌农杆菌中通过去除 47 个氨基酸信号肽进行加工，生成 74 个氨基酸的肽产物。切割的 VirB2 蛋白在根癌农杆菌中进一步环化形成 T 菌毛蛋白，但在大肠杆菌中则不然。 VirB2 菌毛蛋白信号肽酶切割序列的突变会导致异常 T 菌毛蛋白的形成，并严重减弱毒力。在这些突变体中没有观察到T菌毛。讨论了确切的 VirB2 丙菌素裂解和环化在 T 菌毛生物发生和毒力中的潜在作用。
VirB2 propilin is processed by the removal of a 47-amino-acid signal peptide to generate a 74-amino-acid peptide product in both Escherichia coli and Agrobacterium tumefaciens. The cleaved VirB2 protein is further cyclized to form the T pilin in A. tumefaciens but not in E. coli. Mutations in the signal peptidase cleavage sequence of VirB2 propilin cause the formation of aberrant T pilin and also severely attenuate virulence. No T pilus was observed in these mutants. The potential role of the exact VirB2 propilin cleavage and cyclization in T pilus biogenesis and virulence is discussed.