Programmed cell death correlates with virus transmission in a filamentous fungus
Programmed cell death correlates with virus transmission in a filamentous fungus
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DOI：
10.1098/rspb.2002.2148
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发表时间：
2002-11-07
影响因子：
4.7
通讯作者：
Milgroom, MG
中科院分区：
文献类型：
--
作者：
Biella, S;Smith, ML;Milgroom, MG
细胞程序性死亡（PCD）是动植物对病原体防御反应的重要组成部分。在这里，我们报告说，PCD也参与防御真菌病原体。营养体不亲和性是真菌中的自我/非自我识别系统，当不亲和菌株的细胞融合时导致PCD。我们量化了丝状子囊真菌Cryphonectria parasitica中与6个营养体不亲和性（维克）基因相关的细胞死亡频率。细胞死亡频率与维克基因对相同毒株之间病毒传播的影响进行了比较。我们发现细胞死亡和病毒传播之间存在显著的负相关性。我们还表明，细胞死亡的不对称性与病毒传播的不对称性相关;更大的传播发生在与不相容株融合后表现出延迟或罕见PCD的维克基因型中。此外，我们发现，病毒感染可以有一个显着的，株特异性的，积极或消极的影响PCD。vie基因功能和病毒之间的特异性相互作用，沿着细胞死亡和传播之间的相关性，强烈暗示PCD是真菌中宿主介导的病原体防御策略。
Programmed cell death (PCD) is an essential part of the defence response in plants and animals against pathogens. Here, we report that PCD is also involved in defence against pathogens of fungi. Vegetative incompatibility is a self/non-self recognition system in fungi that results in PCD when cells of incompatible strains fuse. We quantified the frequency of cell death associated with six vegetative incompatibility (vic) genes in the filamentous ascomycete fungus Cryphonectria parasitica. Cell death frequencies were compared with the effects of vic genes on transmission of viruses between the same strains. We found a significant negative correlation between cell death and virus transmission. We also show that asymmetry in cell death correlates with asymmetry in virus transmission; greater transmission occurs into vic genotypes that exhibit delayed or infrequent PCD after fusion with an incompatible strain. Furthermore, we found that virus infection can have a significant, strain-specific, positive or negative effect on PCD. Specific interactions between vie gene function and viruses, along with correlations between cell death and transmission, strongly implicate PCD as a host-mediated pathogen defence strategy in fungi.