Gut microbiota profiling in Han Chinese with type 1 diabetes
Gut microbiota profiling in Han Chinese with type 1 diabetes
复制标题
汉族 1 型糖尿病患者肠道微生物群分析。
DOI：
10.1016/j.diabres.2018.04.032
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发表时间：
2018-07-01
影响因子：
5.1
通讯作者：
Fang, Chen
中科院分区：
文献类型：
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作者：
Huang, Yun;Li, Si-Cheng;Fang, Chen
目的：研究中国人群中肠道微生物菌群失调是否与T1 D相关，并探讨肠道微生物菌群组成与临床数据之间的关系。方法：在这项研究中，我们招募了10名健康汉族受试者和12名12岁至33岁的T1 D汉族受试者。收集粪便样本进行DNA提取和16 S rRNA测序，然后通过肠道菌群组成的分析。结果：健康和T1 D受试者之间的细菌群落不同。在门水平上，拟杆菌门和厚壁菌门分别是T1 D患者和健康对照的优势门。线性判别分析（LDA）效应量（LEfSe）算法检测到28个细菌分类学分支，在T1 D患者中显示出统计学差异（13个增加，15个减少）。临床资料与微生物群落丰度的相关性分析显示，粪杆菌丰度与HbA 1c水平呈负相关（Z =-2.614，P = 0.017）。抗胰岛细胞自身抗体的检出数与细菌数呈正相关（Z = 2.531，P = 0.011）和嗜胆菌属（Z = 2.477，P = 0.013），而与链球菌的丰度呈负相关（Z =-2.041，P = 0.041）和瘤胃球菌科（Z =-2.23，P = 0.026）。这些结果表明，与健康受试者相比，中国汉族T1 D患者具有明显不同的肠道微生物群，其特征在于增加的拟杆菌/厚壁菌门比率，粪杆菌丰度与HbA 1c呈负相关，拟杆菌丰度与自身抗体的存在呈正相关。(C)2018爱思唯尔B. V.保留所有权利。
Aims: To investigate whether microbial dysbiosis is associated with T1D in Chinese population and to explore relationships between the composition of gut microbiome and clinical data.Methods: In this study, we recruited 10 healthy and 12 T1D Han Chinese subjects between the ages of 12 to 33. Fecal samples were collected for DNA extraction and 16S rRNA sequencing, followed by analyses of the gut microbiota composition.Results: Bacterial communities differed between healthy and T1D subjects. At the phylum level, Bacteroidetes and Firmicutes are the dominant phyla in T1D patients and healthy controls, respectively. The linear discriminant analysis (LDA) effect size (LEfSe) algorithm detected 28 bacterial taxonomic clades showing statistically differences (13 increased and 15 decreased) in T1D patients. Association analyses of clinical data and microbial community abundance demonstrated that abundances of Faecalibacterium were negatively correlated with HbA1c levels (Z = -2.614, P = 0.017). The numbers of detected anti-islet cell autoantibodies were positively correlated with Bacteriodes (Z = 2.531, P = 0.011) and Bilophila (Z = 2.477, P = 0.013) abundances, while negatively correlated with abundances of Streptococcus (Z = -2.041, P = 0.041) and Ruminococcaceae (Z = -2.23, P = 0.026).Conclusions: These results suggest that Han Chinese T1D patients possess distinctly different gut microbiota, compared to healthy subjects, characterized by increased Bacteroidetes/Firmicutes ratio, negative correlation of Faecalibacterium abundance with HbA1c, and positive correlation of Bacteroides abundance with the presence of autoantibodies. (C) 2018 Elsevier B.V. All rights reserved.