Effects of osteoblast-like cell seeding on mechanical properties of porous composite scaffolds
Effects of osteoblast-like cell seeding on mechanical properties of porous composite scaffolds
复制标题
成骨细胞样细胞接种对多孔复合支架力学性能的影响
DOI：
10.1007/978-3-642-14515-5_313
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Todo
中科院分区：
文献类型：
--
作者：
T. Arahira;M. Todo
在组织工程技术中，聚合物-陶瓷或聚合物-聚合物复合材料已被认为是具有机械稳定性、生物相容性、细胞增殖和易加工性的先进支架。然而，这种复合支架的力学性能和细胞增殖行为之间的关系还没有得到澄清。本研究对聚对苯二甲酸乙二醇酯（PET）纤维/胶原和β-磷酸三钙（β-TCP）/明胶两种复合支架进行了研究。将MC 3 T3-E1细胞在适当的条件下在这些支架中培养。然后定期进行压缩测试以评估压缩弹性模量。结果发现，含有细胞的支架的模量随着细胞培养时间的增加而增加。值得注意的是，具有细胞的β-TCP/明胶的模量比具有细胞的PET纤维/胶原蛋白的模量大约7倍。
In tissue engineering technology, polymer–ceramics or polymer–polymer composites have been considered as advanced scaffolds having mechanical stability, biocompatibility, cell proliferation, and easy processability. However, the relationship between the mechanical properties and the cell proliferation behavior of such composite scaffolds has not been clarified yet. In this study, two types of composite scaffolds, poly(ethylene terephthalate) (PET) fiber/collagen andβ-tricalcium phosphate (β-TCP)/gelatin scaffolds, were investigated. MC3T3-E1 cells were cultured in these scaffolds under appropriate conditions. Compression tests were then periodically conducted to evaluate the compressive elastic modulus. It was found that the modulus of the scaffolds containing cells increased with the cell culture period. It is noted that the modulus of theβ-TCP/gelatin with cells was approximately seven times larger than that of the PET fiber/collagen with cells.