Labeling of platelet surface proteins with 125I by the iodogen method.
Labeling of platelet surface proteins with 125I by the iodogen method.
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DOI：
10.1016/0003-2697(83)90664-4
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发表时间：
1983-04
影响因子：
2.9
通讯作者：
G. Tuszynski;L. Knight;E. Kornecki;S. Srivastava
中科院分区：
文献类型：
--
作者：
G. Tuszynski;L. Knight;E. Kornecki;S. Srivastava
描述了血小板悬浮液的 125 I-碘化程序。该过程使用碘，一种类似于氯胺-T 的固相氧化剂。在多种条件下对血小板进行标记，包括在存在0.1%白蛋白的情况下，并且显示125 I的掺入率为7%至28%。在低血小板浓度（3–5 × 108 血小板/ml）、短反应时间（15 分钟）以及使用涂有 100 μg 碘原的 2 ml 玻璃瓶中获得最佳标记结果。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和随后的放射自显影对标记的血小板蛋白进行分析表明，通过该方法标记的主要蛋白质条带与通过乳过氧化物酶方法标记的蛋白质条带相同。在低血小板浓度下，碘化程序可提供两倍的碘掺入量。
A procedure for the125I-iodination of platelet suspensions is described. The procedure utilizes Iodogen, a solid-phase oxidizing agent similar to chloramine-T. Platelets were labeled under a variety of conditions, including in the presence of 0.1% albumin, and showed between 7 and 28% incorporation of125I. Best labeling results were obtained at low platelet concentrations (3–5 × 108platelets/ml), short reaction times (15 min), and with 2-ml glass vials coated with 100 μg of Iodogen. Analysis of the labeled platelet proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography revealed that the same major protein bands were labeled by this procedure as were labeled by the lactoperoxidase procedure. At low platelet concentrations, the Iodogen procedure gives twice the amount of iodine incorporation.