Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)
Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)
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DOI：
10.1271/bbb.130064
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发表时间：
2013-05-01
影响因子：
1.6
通讯作者：
Hirata, Dai
中科院分区：
文献类型：
--
作者：
Kume, Kazunori;Kubota, Shunsuke;Hirata, Dai
在真核生物中，细胞形态发生与细胞周期协调调节。在裂殖酵母中，形态发生网络莫尔（morphogenesis Orb 6 network）由5个保守蛋白质Pmo 25、Nak 1、Mor 2、Orb 6和Mob 2组成，并且对于胞质分裂后的细胞极性控制和细胞分离是必需的。在这里，我们表明，保守的富含亮氨酸的重复蛋白Lrp 1是细胞形态发生所需的一个新的公认的组成部分的莫尔。Lrp 1在其N-末端有4个富含亮氨酸的重复序列，是芽殖酵母Sog 2的同源物，Sog 2是RAM网络（Ace 2活性和细胞形态发生的调节）的组成部分。Lrp 1对细胞生长和细胞形态发生都是必不可少的，其他莫尔组分也是如此。Lrp 1定位于SPBs（纺锤体极体，酵母相当于动物中心体）在整个细胞周期和细胞质分裂期间的中间环。Lrp 1与Nak 1相互作用，对Orb 6激酶活性很重要。因此，Lrp 1被证明在细胞形态发生中Orb 6的上游起作用。
In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis.