A Microinjection Method for Infecting the Planthopper Sogatella furcifera (Hemiptera: Delphacidae) with the Southern Rice Black-Streaked Dwarf Virus
A Microinjection Method for Infecting the Planthopper Sogatella furcifera (Hemiptera: Delphacidae) with the Southern Rice Black-Streaked Dwarf Virus
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DOI：
10.1093/jee/toz106
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发表时间：
2019-08
影响因子：
2.2
通讯作者：
Kui Hu;Lin Qiu;Yurong Zhang;Yu Du;Hua-liang He;W. Ding;Youzhi Li
中科院分区：
文献类型：
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作者：
Kui Hu;Lin Qiu;Yurong Zhang;Yu Du;Hua-liang He;W. Ding;Youzhi Li
摘要南方水稻黑条矮缩病毒（SRBSDV）对水稻造成严重的经济损失。该病毒通过灰飞虱Sogatella furcifera（Horváth）以持久、循环和繁殖的方式传播到水稻植株。研究人员目前缺乏合适的方法来测定体外SRBSDV的活性并全年保存病毒。采用微量注射法将SRBSDV提取液直接注射入S.白背蕨随后通过逆转录-聚合酶链反应（RT-PCR）检测到SRBSDV在5d后超过56.7%的昆虫中被检测到，并且60%的健康水稻植株也被这些昆虫取食而成为SRBSDV感染。此外，用在-80 ° C冷冻220 d的SRBSDV感染水稻植株的提取物注射稻飞虱，导致63.3%变成带毒。这些结果表明，SRBSDV可以成功地传播到S。感染SRBSDV的水稻植株在-80 ° C冷冻220 d后，其提取物中仍含有足够的活性SRBSDV。furcifera。我们提供了一种新的方法来保存SRBSDV全年注射S。白背飞虱与SRBSDV提取物的混合物。
Abstract The southern rice black-streaked dwarf virus (SRBSDV) causes significant economic damage to rice crops. This virus is transmitted to rice plants by the planthopper Sogatella furcifera (Horváth) in a persistent, circular, and propagative manner. Researchers currently lack suitable methods for assaying the activity of SRBSDV in vitro and preserving the virus all year. We used a microinjection method to directly inject SRBSDV extracts into the hemocoel of S. furcifera nymphs. SRBSDV was subsequently detected by Reverse Transcription-Polymerase Chain Reaction in more than 56.7% of the insects after 5 d and 60% of healthy rice plants fed by these insects also became SRBSDV infected. Moreover, injecting planthopper with an extract of SRBSDV-infected rice plant that had been frozen at –80°C for 220 d caused 63.3% to become viruliferous. These results indicate that SRBSDV can be successfully transmitted to S. furcifera by microinjection, and that extracts of SRBSDV-infected rice plants frozen at –80°C for 220 d still contain sufficient active SRBSDV to infect S. furcifera. We provide a novel way to preserve SRBSDV all year by injecting S. furcifera with the SRBSDV extract.