Analysis of Fluorescent Proteins with a Nanoparticle Probe.
Analysis of Fluorescent Proteins with a Nanoparticle Probe.
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用纳米颗粒探针分析荧光蛋白。
DOI：
10.1021/jz201547x
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Schweikert,EmileA
中科院分区：
文献类型：
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作者：
Fernandez-Lima,FranciscoA;Eller,MichaelJ;Debord,JDaniel;Levy,MichaellaJ;Verkhoturov,StanislavV;Della-Negra,Serge;Schweikert,EmileA
这封信介绍了高能量，单纳米粒子探针的第一个应用（例如，520 keV Au 4002 nm NP）用于表征含有荧光蛋白的表面（例如，GFP变体）通过其共发射的光子、电子和二次离子信号。NP诱导的蛋白质发光随着NP入射能量的增加而增加，其起源于NP撞击，并通过电子能量转移转移到蛋白质荧光团。多电子发射观察到每个单一的NP的影响，和它们的分布是特定的目标形态和组合物。在单个NP冲击下观察到由2-7个氨基酸肽组成的蛋白质亚基的片段离子，其可以与相对于冲击位点的随机蛋白质取向相关（例如，蛋白质的外层或“皮肤”）。
This Letter presents the first application of high-energy, single nanoparticle probes (e.g., 520 keV Au4002 nm NP) in the characterization of surfaces containing fluorescent proteins (e.g., GFP variants) by their coemitted photon, electron and secondary ion signals. NP-induced protein luminescence increases with the NP incident energy, is originated by the NP impact, and is transferred to the protein fluorophor via electronic energy transfer. Multielectron emission is observed per single NP impacts, and their distributions are specific to the target morphology and composition. Fragment ions of protein subunits consisting of 2–7 amino acid peptides are observed under individual NP impacts that can be correlated to the random protein orientation relative to the impact site (e.g., outer layer or “skin” of the protein).