Characterization of three new enterococcal species, Enterococcus sp nov CDC PNS-E2, Enterococcus sp nov CDC PNS-E3, and Enterococcus sp nov CDC PNS-E3, isolated from human clinical specimens
Characterization of three new enterococcal species, Enterococcus sp nov CDC PNS-E2, Enterococcus sp nov CDC PNS-E3, and Enterococcus sp nov CDC PNS-E3, isolated from human clinical specimens
复制标题
DOI：
10.1128/jcm.42.3.1192-1198.2004
复制
发表时间：
2004-03-01
影响因子：
9.4
通讯作者：
Facklam, RR
中科院分区：
文献类型：
--
作者：
Carvalho, MDS;Steigerwalt, AG;Facklam, RR
作为参考实验室，疾病控制和预防中心（CDC）的链球菌实验室经常被要求确认不常见或难以识别的过氧化氢酶阴性革兰氏阳性球菌的身份。为了完成这些微生物的精确鉴定，我们系统地应用了全细胞蛋白质谱分析（WCPP）和DNA-DNA再结合实验，结合常规的生理测试。使用这种方法，我们最近集中在三个菌株的特性类似的生理群I（菌株SS-1730），II（菌株SS-1729），和IV（菌株SS-1728）的肠球菌物种。从人血液中分离到两株，从人脑组织中分离到一株。生理学检测的结果不一致，不足以将菌株纳入任何已知的肠球菌菌种中。在其中一株菌株（SS-1729）中检测到对万古霉素的耐药性。WCPP的分析显示，每种菌株的特征都是独特的，与任何先前描述的肠球菌属物种的特征都不相似。16 S核糖体DNA（rDNA）测序结果揭示了三个新的分类群内的肠球菌属。DNA-DNA相关性实验结果与WCPP分析和16 SrDNA测序结果一致，与25种已知肠球菌的同源性均低于70%。总体而言，结果表明，这三种菌株构成了从人类临床来源鉴定的三种新的肠球菌属，包括一种携带vanA基因的肠球菌属。这些分离株暂时被命名为肠球菌属新种。（CDC PNS-E1），模式菌株SS-1728（T）（= ATCC BAA-780（T）= CCUG 47860（T））;肠球菌属CDC PNS-E2，模式菌株SS-1729（T）（= ATCC BAA-781（T）= CCUG 47861（T））;和肠球菌属CDC PNS-E3，模式菌株SS-1730（T）（= ATCC BAA-782（T）= CCUG 47862（T））。
As a reference laboratory, the Streptococcus Laboratory at the Centers for Disease Control and Prevention (CDC) is frequently asked to confirm the identity of unusual or difficult-to-identify catalase-negative, grampositive cocci. In order to accomplish the precise identification of these microorganisms, we have systematically applied analysis of whole-cell protein profiles (WCPP) and DNA-DNA reassociation experiments, in conjunction with conventional physiological tests. Using this approach, we recently focused on the characterization of three strains resembling the physiological groups I (strain SS-1730), II (strain SS-1729), and IV (strain SS-1728) of enterococcal species. Two strains were isolated from human blood, and one was isolated from human brain tissue. The results of physiological testing were not consistent enough to allow confident inclusion of the strains in any of the known enterococcal species. Resistance to vancomycin was detected in one of the strains (SS-1729). Analysis of WCPP showed unique profiles for each strain, which were not similar to the profiles of any previously described Enterococcus species. 16S ribosomal DNA (rDNA) sequencing results revealed three new taxa within the genus Enterococcus. The results of DNA-DNA relatedness experiments were consistent with the results of WCPP analysis and 16S rDNA sequencing, since the percentages of homology with all 25 known species of Enterococcus were lower than 70%. Overall, the results indicate that these three strains constitute three new species of Enterococcus identified from human clinical sources, including one that harbors the vanA gene. The isolates were provisionally designated Enterococcus sp. nov. CDC Proposed New Species of Enterococcus 1 (CDC PNS-E1), type strain SS-1728(T) (= ATCC BAA-780(T) = CCUG 47860(T)); Enterococcus sp. nov. CDC PNS-E2, type strain SS-1729(T) (= ATCC BAA-781(T) = CCUG 47861(T)); and Enterococcus sp. nov. CDC PNS-E3, type strain SS-1730(T) (= ATCC BAA-782(T) = CCUG 47862(T)).