Biomechanical properties of vaginal tissue. Part 1: New experimental protocol
Biomechanical properties of vaginal tissue. Part 1: New experimental protocol
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DOI：
10.1016/j.juro.2007.03.040
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发表时间：
2007-07-01
影响因子：
6.6
通讯作者：
Cosson, Michel
中科院分区：
文献类型：
--
作者：
Rubod, Chrystele;Boukerrou, Malik;Cosson, Michel
目的：我们建立了一个可靠的实验方案，以表征阴道组织的生物力学性能，并保证良好的测试replication.Materials和方法：由于大量的组织需要建立的协议，我们工作的母羊根据动物伦理法律。为了研究母羊阴道组织的力学性能，我们采用恒定变形率的单向拉伸试验。在不同实验条件下进行破裂测试，分析各条件的影响。结果：组织进行了详尽的测试。研究的参数包括取样、冷冻、保存条件、湿度和温度（引诱阴道组织试验）以及试验期间的变形率。如前所述，阴道组织是各向异性的，并且之前已经对收集物进行了测试。我们注意到，在单向拉伸试验期间，冷冻组织对组织的机械响应没有影响。我们定义的实验条件（温度，湿度和变形率），使我们能够有可重复的tests.Conclusions：结果和分析，使我们能够确定最佳的参考协议。
Purpose: We established a reliable experimental protocol to characterize the biomechanical properties of vaginal tissue and guarantee good test repeatability.Materials and Methods: Because of the large quantities of tissue required to establish the protocol, we worked On ewes according to animal ethics laws. To study the mechanical properties of ewe vaginal tissue we used unidirectional tension tests at a constant deformation rate. Rupture tests were performed under different experimental conditions to analyze the influence of each condition.Results: Tissue underwent exhaustive tests. The parameters studied were sampling, freezing, preservation conditions, hygrometry and temperature (luring vaginal tissue tests, and the rate of deformation during tests. As previously, noted, vaginal tissue is anisotropic and the collection has been tested previously. We noted that freezing tissue had no consequences on the mechanical response of tissue during unidirectional tension testing. The experimental conditions that we defined (temperature, hygrometry and rate of deformation) allowed us to have reproducible tests.Conclusions: Results and analyses allowed us to determine the best reference protocol.