Prenatal gonadal steroids affect adult spatial behavior, CA1 and CA3 pyramidal cell morphology in rats
Prenatal gonadal steroids affect adult spatial behavior, CA1 and CA3 pyramidal cell morphology in rats
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DOI：
10.1006/hbeh.1998.1477
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发表时间：
1998-10-01
影响因子：
3.5
通讯作者：
Sengelaub, DR
中科院分区：
文献类型：
--
作者：
Isgor, C;Sengelaub, DR
本研究评估了产前雄激素和雌激素暴露是否影响成年人的空间学习和海马形态。在成年期评估水迷宫性能、CA 1和CA 3锥体细胞野以及齿状回颗粒细胞层（DG-GCL）形态（70天以上）雄性、雌性、雄激素给药（丙酸睾酮，TP或丙酸二氢睾酮，DHTP）雌性（2-4 mg/天）、苯甲酸雌二醇（EB）处理的雌性（100 μ g/天）和用抗雄激素氟替卡松处理的雄性（8 mg/天）。妊娠大鼠在胚胎第16天至出生期间每日（sc）注射一次;所有幼仔均通过剖宫产分娩。氟他胺处理的雄性动物在分娩时被阉割，成年阉割用于控制激活效应。在水迷宫中观察到对类固醇敏感的性别差异，有利于男性。雄鼠的CA 1和CA 3锥体细胞野体积和索马体积均大于雌鼠，且氟替卡松处理使雄鼠雌性化。TP和EB，但不是DHTP，雄性化的CA 1锥体细胞场体积和神经元索马大小; CA 3在TP和DHTP处理的女性中雄性化，而EB是无效的。未观察到细胞密度、数量或DG-GCL体积或成人激素水平的影响。因此，产前雄激素和雌激素的影响性别差异，在成人空间导航和发挥不同的影响，CA 1和CA 3锥体细胞形态。因此，除了以前报道的产后组件，也有一个产前组件的关键时期，性腺类固醇组织的神经机制的性别差异在成人的空间能力，（C）1998年学术出版社。
The present study assessed whether prenatal androgen and estrogen exposure affected adult spatial learning and hippocampal morphology. Water maze performance, the CA1 and CA3 pyramidal cell field, and the dentate gyrus-granule cell layer (DG-GCL) morphology were assessed at adulthood (70+ days of age) in males, females, androgen-treated (testosterone propionate, TP, or dihydrotestosterone propionate, DHTP) females (2-4 mg/day), estradiol benzoate (EB)-treated females (100 mu g/day), and males treated with the antiandrogen flutamide (8 mg/day). Pregnant rats were injected daily (sc) between Embryonic Day 16 and birth; all pups were delivered by cesarean section. Flutamide-treated males were castrated upon delivery, and adult castrates were used to control for activational effects. Steroid-sensitive sex differences were observed in water maze performance in favor of males. Males had larger CA1 and CA3 pyramidal cell field volumes and soma sizes than females, which were feminized with flutamide treatment. TP and EB, but not DHTP, masculinized CA1 pyramidal cell field volume and neuronal soma size; CA3 was masculinized in both TP- and DHTP-treated females, while EB was ineffective. No effects were observed in cell density, number, or DG-GCL volume or due to adult hormone levels. Thus, prenatal androgens and estrogen influence sex differences in adult spatial navigation and exert differential effects on CA1 and CA3 pyramidal cell morphology. Hence, in addition to the previously reported postnatal component, there is also a prenatal component to the critical period in which gonadal steroids organize the neural mechanisms underlying sex differences in adult spatial ability, (C) 1998 Academic Press.