The binding of nuclear factors to the as-1 element in the CaMV 35S promoter is affected by cytosine methylation in vitro
The binding of nuclear factors to the as-1 element in the CaMV 35S promoter is affected by cytosine methylation in vitro
复制标题
DOI：
10.1055/s-2006-924633
复制
发表时间：
2007-05-01
期刊：
影响因子：
3.9
通讯作者：
Hellens, R. R.
中科院分区：
文献类型：
--
作者：
Kanazawa, A.;O'Dell, M.;Hellens, R. R.
转录基因沉默（TGS）通常与受影响启动子中胞嘧啶甲基化水平升高有关。以矮牵牛花提取物为载体，研究了花椰菜花叶病毒（CaMV） 35S启动子序列甲基化对其与细胞核内因子结合的影响。在CaMV 35S启动子包含as-1元件的区域和- 345和- 208之间的区域检测到特异性的dna -蛋白相互作用。蛋白因子(s)与as-1元素的结合受到胞嘧啶甲基化的影响，而与-345和-208之间区域的结合则不受影响。结果表明，as-1元素的胞嘧啶甲基化可能影响CaMV 35S启动子的活性。
Transcriptional gene silencing (TGS) is often associated with an increased level of cytosine methylation in the affected promoters. The effect of methylation of the cauliflower mosaic virus (CaMV) 35S promoter sequence on its binding to factors present in the nuclei was analyzed by electrophoretic mobility shift assays using extracts of petunia flowers. Specific DNA-protein interactions were detected in the region of the CaMV 35S promoter that contains the as-1 element and the region between - 345 and - 208. The binding of protein factor(s) to the as-1 element was influenced by cytosine methylation, whereas the binding to the region between -345 and -208 was unaffected. The results suggest that cytosine methylation of the as-1 element potentially affects the activity of the CaMV 35S promoter.