Comparative study for the virulence of Mycobacterium avium isolates from patients with nodular-bronchiectasis-and cavitarytype diseases
Comparative study for the virulence of Mycobacterium avium isolates from patients with nodular-bronchiectasis-and cavitarytype diseases
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结节性支气管扩张症和空洞型疾病患者鸟分枝杆菌毒力的比较研究
DOI：
10.1007/s10096-010-0930-2
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Tomioka H
中科院分区：
文献类型：
--
作者：
Tatano Y;Yasumoto K;Shimizu T;Sano C;Sato K;Yano S;Takeyama H;Tomioka H
鸟分枝杆菌（Mav）肺部感染，称为结节性支气管扩张（NB）型。肺炎复合体（MAC）疾病在全球范围内呈上升趋势。为了阐明是否存在引起nb型疾病而非空洞（CA）型疾病的Mav异常种群，我们基于不同类型人类细胞的细胞内生长，比较了nb型（NB-Mav）和CA型（CA-Mav）疾病患者分离的Mav的毒力。比较NB-Mav和CA-Mav各5株菌株在不同类型人源性培养细胞中的侵袭性和细胞内复制能力。平均而言，两种类型的Mav分离株在巨噬细胞和肺上皮细胞中复制的能力相似。此外，它们在诱导巨噬细胞产生活性氮中间体和活性氧中间体以及对抗菌分子的敏感性方面表现出相似的能力。因此，从NB-MAC病和CA-MAC病分离的Mav菌株在对人巨噬细胞和肺细胞的感染性方面似乎没有本质上的毒力差异。这些发现表明，进一步研究基于宿主免疫条件而非MAC生物本身的致病性质建立nb型MAC疾病的机制具有重要意义。
Mycobacterium avium(Mav) lung infections, called nodular-bronchiectasis (NB)-typeM. aviumcomplex (MAC) disease, are globally increasing. To elucidate whether there are unusual populations of Mav, causing NB-type disease rather than cavitary (CA)-type disease, we compared the virulence of Mav isolates from patients with NB-type (NB-Mav) and those from CA-type (CA-Mav) diseases, based on intracellular growth in various types of human cells. Five strains each of NB-Mav and CA-Mav were compared with each other for their invasiveness and ability to intracellularly replicate in various types of cultured cells of human origin. The two types of Mav isolates showed a similar ability, on average, to replicate in macrophages and lung epithelial cells. Moreover, they showed a similar ability to induce the production of reactive nitrogen intermediates and reactive oxygen intermediates by macrophages and susceptibility to antimicrobial molecules. Therefore, it appears that there is no essential difference in virulence in terms of infectivity to human macrophages and lung cells between Mav strains isolated from NB-MAC disease and those from CA-MAC disease. These findings indicate the importance of further studies to elucidate the mechanism for the establishment of NB-type MAC diseases based on host immunological conditions rather than the pathogenic nature of MAC organisms themselves.