Alleviation of Copper-Induced Oxidative Damage in Chlamydomonas reinhardtii by Carbon Monoxide
Alleviation of Copper-Induced Oxidative Damage in Chlamydomonas reinhardtii by Carbon Monoxide
复制标题
DOI：
10.1007/s00244-010-9602-6
复制
发表时间：
2011-08
影响因子：
4
通讯作者：
Q. Zheng;Qian Meng;Y. Wei;Z. Yang
中科院分区：
文献类型：
--
作者：
Q. Zheng;Qian Meng;Y. Wei;Z. Yang
一氧化碳（CO）是植物和动物体内的内源性气体分子。最近的研究表明，它是调节植物生长和发育的许多方面的最重要的细胞组分之一。然而，CO是否调节绿色藻类对重金属毒性的适应性反应尚不清楚。本研究探讨了CO在真核藻类莱茵衣藻中调节铜诱导的氧化应激中的作用。用5 μM CO预处理30 min，然后暴露于5 μM Cu（II）4 d，显示毒性减弱。CO改善藻类的生长与减少脂质过氧化和增加叶绿素积累。活性氧组化染色证实了CO的有益作用。此外，5 μM CO处理提高了过氧化氢酶与Cu的活性。然而，减少超氧化物歧化酶的活性，观察到在CO + Cu处理的藻类相比，控制（单独的Cu处理的活性）。在相同条件下，抗坏血酸过氧化物酶活性无明显变化。这些结果表明，CO可以在调节藻类对铜胁迫的响应中发挥重要作用。
Carbon monoxide (CO) is an endogenous gaseous molecule in plants and animals. Recent studies have shown that it is one of the most essential cellular components regulating many aspects of plant growth and development. However, whether CO regulates the green algae adaptive response to heavy metal toxicity is unknown. The present study investigated the role of CO in regulating Cu-induced oxidative stress in eukaryotic algaeChlamydomonas reinhardtii. Cells pretreated with 5 μM CO for 30 min and followed by exposure to 5 μM Cu(II) for 4 days showed attenuated toxicity. The CO-improved growth of algae was correlated with reduced lipid peroxidation and increased chlorophyll accumulation. The beneficial effect of CO was confirmed by histochemical staining with reactive oxygen species. Further, treatment with 5 μM CO increased the activity of catalase with Cu. However, a reduced superoxide dismutase activity was observed in the CO + Cu-treated algae compared to the control (activity of Cu treatment alone). Under the same condition, the activity of ascorbate peroxidase was not significantly changed. These results suggest that CO can play an important role in regulating the response of algae to Cu stress.