Characterization of the complete mitochondrial genome of the giant silkworm moth, Eriogyna pyretorum (Lepidoptera: Saturniidae).

Characterization of the complete mitochondrial genome of the giant silkworm moth, Eriogyna pyretorum (Lepidoptera: Saturniidae).
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巨型蚕蛾 Eriogynapyretorum(鳞翅目:Saturniidae)完整线粒体基因组的表征

DOI:
10.7150/ijbs.5.351
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发表时间:
2009-05-22
影响因子:
9.2
通讯作者:
Wei ZJ
Wei ZJ
中科院分区:
生物学2区
文献类型:
--
作者:
Jiang ST;Hong GY;Yu M;Li N;Yang Y;Liu YQ;Wei ZJ

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结果表明,蜡蝉(EriogynapypyrumL.)线粒体基因组全长15,327 bp,包括13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和一个控制区。PCG的排列与其他已测序的鳞翅目昆虫相同。E.腐霉有丝分裂基因组为轻微阴性(-0.031),表明Ts的出现多于As。E.腐霉有丝分裂基因组也偏向于A + T核苷酸(80.82%)。除了细胞色素c氧化酶亚基1和2(cox 1和cox 2)外,所有PCG均由ATN密码子启动。13个PCG中有2个含有T.所有的tRNA基因都有一个典型的三叶草结构的线粒体tRNA,除了trnS 1(AGN)和trnS 2(UCN)。对现有鳞翅目昆虫的系统发育分析支持了目前基于形态学的假设,即鳞翅目总科、尺蛾总科、背齿亚目、凤蝶总科和螟蛾总科是单系的。如前所述,天蚕科(Antheraea pernyi,Antheraea yamai,E. pyramidum和Caligula boisduvalii)形成一个类群。
The complete mitochondrial genome (mitogenome) of Eriogyna pyretorum (Lepidoptera: Saturniidae) was determined as being composed of 15,327 base pairs (bp), including 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The arrangement of the PCGs is the same as that found in the other sequenced lepidopteran. The AT skewness for the E. pyretorum mitogenome is slightly negative (-0.031), indicating the occurrence of more Ts than As. The nucleotide composition of the E. pyretorum mitogenome is also biased toward A + T nucleotides (80.82%). All PCGs are initiated by ATN codons, except for cytochrome c oxidase subunit 1 and 2 (cox1 and cox2). Two of the 13 PCGs harbor the incomplete termination codon by T. All tRNA genes have a typical clover-leaf structure of mitochondrial tRNA, with the exception of trnS1(AGN) and trnS2(UCN). Phylogenetic analysis among the available lepidopteran species supports the current morphology-based hypothesis that Bombycoidea, Geometroidea, Notodontidea, Papilionoidea and Pyraloidea are monophyletic. As has been previously suggested, Bombycidae (Bombyx mori and Bombyx mandarina), Sphingoidae (Manduca sexta) and Saturniidae (Antheraea pernyi, Antheraea yamamai, E. pyretorum and Caligula boisduvalii) formed a group.