c-Maf and MafB transcription factors are differentially expressed in Huxley's and Henle's layers of the inner root sheath of the hair follicle and regulate cuticle formation
c-Maf and MafB transcription factors are differentially expressed in Huxley's and Henle's layers of the inner root sheath of the hair follicle and regulate cuticle formation
复制标题
DOI：
10.1016/j.jdermsci.2009.12.011
复制
发表时间：
2010-03-01
影响因子：
4.6
通讯作者：
Kataoka, Kohsuke
中科院分区：
文献类型：
--
作者：
Miyai, Masashi;Tanaka, Yoshihiro G.;Kataoka, Kohsuke
背景：哺乳动物皮肤的毛囊由一组同心的上皮细胞层组成。内根鞘 (IRS) 围绕皮肤表面下硬化的毛干，分为三层，称为 IRS 角质层。赫胥黎层和亨利层。 IRS 在毛道中形成毛囊壁，帮助引导毛干的发育。 c-Maf和MafB是Maf转录因子家族的成员，在各种组织的发育过程和细胞类型特异性基因表达中发挥着重要作用。目的：本研究的目的是揭示c-Maf和MafB在毛囊中的表达模式和功能作用。方法：我们利用层特异性标记物对小鼠皮肤切片进行免疫荧光染色，确定c-Maf和MafB表达的精确位置。我们还使用扫描电子显微镜分析了 c-maf-和 mafB-null 小鼠（分别为 c-maf(-/-) 和 mafB(-/-)）的胡须。结果：c-Maf 和 MafB 在 IRS 的 Huxley 层和 Henle 层中差异表达。扫描电子显微镜分析显示 c-maf(-/-) 和 mafB(-/-) 小鼠胡须的角质层图案不规则。 mafB(-/-)小鼠的角质层也比野生型小鼠薄。结论：c-Maf和MafB以谱系限制性方式在IRS层中表达，并参与毛发形态发生。 (C) 2009 年日本皮肤病研究学会。由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Background: The hair follicle of mammalian skin consists of a group of concentric epithelial cell layers. The inner root sheath (IRS), which surrounds the hardening hair shaft beneath the skin surface, is subdivided into three layers, termed the cuticle of the IRS. Huxley's layer, and Henle's layer. The IRS forms a follicular wall in the hair canal and helps guide the developing hair shaft. c-Maf and MafB, members of the Maf family of transcription factors, play important roles in the developmental processes of various tissues and in cell type-specific gene expression.Objective: The aim of this study is to reveal the pattern of expression and functional roles of c-Maf and MafB in the hair follicle.Methods: We determined the precise location of c-Maf and MafB expression using immunofluorescent staining of mouse skin sections with layer-specific markers. We also analyzed whiskers of c-maf- and mafB-null mice (c-maf(-/-) and mafB(-/-), respectively) using scanning electron microscopy.Results: c-Maf and MafB were differentially expressed in the Huxley's and Henle's layers of the IRS. Scanning electron microscopic analysis showed irregular cuticle patterning of whiskers of c-maf(-/-) and mafB(-/-) mice. The cuticles of mafB(-/-) mice were also thinner than those of wild-type mice.Conclusion: c-Maf and MafB are expressed in the IRS layers in a lineage-restricted manner and are involved in hair morphogenesis. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.