Two forms of cerebellar glial cells interact differently with neurons in vitro.
Two forms of cerebellar glial cells interact differently with neurons in vitro.
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DOI：
10.1083/jcb.98.1.193
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发表时间：
1984-01
期刊：
影响因子：
--
通讯作者：
Mason CA
中科院分区：
文献类型：
--
作者：
Hatten ME;Liem RK;Mason CA
通过间接免疫细胞化学染色，用纯化的胶质丝蛋白、半乳糖脑苷和尼罗糖蛋白抗血清在体外研究了从出生后早期小鼠小脑组织分离的神经元和胶质细胞之间的特异性相互作用。两种类型的细胞用针对纯化胶质丝蛋白的抗血清染色。第一种细胞的特点是细胞体直径9微米，突起长130-150微米，通常有两到三个与之相关的神经元，类似于伯格曼神经胶质。第二种细胞的胞体稍大，胞臂明显较短，胞臂内嵌有几十个神经元细胞，类似于颗粒层的星形胶质细胞。纯化半乳糖脑苷单克隆抗血清染色显示培养物中存在未成熟的少突胶质细胞。这些胶质细胞约占培养细胞总数的2%，与星形胶质细胞相反，它们没有与神经元形成特定的接触。用两种神经元标记物（纯化尼罗糖蛋白和破伤风毒素的抗血清）染色显示，大多数与假定的星形胶质细胞相关的细胞是小神经元（5-8微米）。在培养1-2 d后，一些染色的神经元具有非常细的短突起。几乎所有大于10-20微米长的突起都起源于胶质细胞。电子显微镜也显示了培养物中存在两种形式的星形胶质细胞，每一种都对小脑神经元有不同的组织影响。与星形胶质细胞相关的大多数神经元是颗粒神经元，尽管有时也有一些较大的神经元与它们相关。延时视频显微镜显示沿伯格曼样星形胶质细胞臂有广泛的细胞迁移（约10微米/小时）。相反，细胞不会沿着星形胶质细胞样臂移动，而是在分支点或分支点附近保持静止。生长锥活动，核周细胞搏动运动，神经胶质和神经元突起膜皱褶也可见。
Specific interactions between neurons and glia dissociated from early postnatal mouse cerebellar tissue were studied in vitro by indirect immunocytochemical staining with antisera raised against purified glial filament protein, galactocerebroside, and the NILE glycoprotein. Two forms of cells were stained with antisera raised against purified glial filament protein. The first, characterized by a cell body 9 microns diam and processes 130-150 microns long, usually had two to three neurons associated with them and resembled Bergmann glia. The second had a slightly larger cell body with markedly shorter arms among which were nestled several dozen neuronal cells, and resembled astrocytes of the granular layer. Staining with monoclonal antisera raised against purified galactocerebroside revealed the presence of immature oligodendroglia in the cultures. These glial cells constituted approximately 2% of the total cell population in the cultures and, in contrast to astroglia, did not form specific contacts with neurons. Staining with two neuronal markers, antisera raised against purified NILE glycoprotein and tetanus toxin, revealed that most cells associated with presumed astroglia were small neurons (5-8 microns). After 1-2 d in culture, some stained neurons had very fine, short processes. Nearly all of the processes greater than 10-20 micron long were glial in origin. Electron microscopy also demonstrated the presence of two forms of astroglia in the cultures, each with a different organizing influence on cerebellar neurons. Most neurons associated with astroglia were granule neurons, although a few larger neurons sometimes associated with them. Time-lapse video microscopy revealed extensive cell migration (approximately 10 microns/h) along the arms of Bergmann-like astroglia. In contrast, cells did not migrate along the arms of astrocyte-like astroglia, but remained stationary at or near branch points. Growth cone activity, pulsating movements of cell perikarya, and ruffling of the membranes of glial and neuronal processes were also seen.