Depth-resolved optical imaging of transmural electrical propagation in perfused heart
Depth-resolved optical imaging of transmural electrical propagation in perfused heart
复制标题
DOI：
10.1364/oe.15.017827
复制
发表时间：
2007-12-24
期刊：
影响因子：
3.8
通讯作者：
Pertsov, Arkady
中科院分区：
文献类型：
--
作者：
Hillman, Elizabeth M. C.;Bernus, Olivier;Pertsov, Arkady
我们提出了一个研究的三维（3D）传播的电波在心脏壁使用层流光学断层扫描（LOT）。光学成像对比度由电压敏感染料提供，其荧光报告膜电位的变化。我们研究了在Langendorf灌注的大鼠心脏的右心室的电波的跨壁传播动力学，无论是由endo-endoscopic或epi-endoscopic起搏。以667 Hz的有效帧速率获取3D图像。我们比较我们的实验结果的电跨壁传播的数学模型。我们证明，LOT可以清楚地解决电波在心脏壁内的传播方向，并且观察到的动态与大鼠心室组织中的电传播模型一致。(c)2007年，美国光学学会。
We present a study of the 3-dimensional (3D) propagation of electrical waves in the heart wall using Laminar Optical Tomography ( LOT). Optical imaging contrast is provided by a voltage sensitive dye whose fluorescence reports changes in membrane potential. We examined the transmural propagation dynamics of electrical waves in the right ventricle of Langendorf perfused rat hearts, initiated either by endo-cardial or epi-cardial pacing. 3D images were acquired at an effective frame rate of 667Hz. We compare our experimental results to a mathematical model of electrical transmural propagation. We demonstrate that LOT can clearly resolve the direction of propagation of electrical waves within the cardiac wall, and that the dynamics observed agree well with the model of electrical propagation in rat ventricular tissue. (c) 2007 Optical Society of America.