Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts
Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts
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DOI：
10.1016/j.intimp.2011.01.009
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发表时间：
2011-06-01
影响因子：
5.6
通讯作者：
Matsuo, Takashi
中科院分区：
文献类型：
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作者：
Hosokawa, Yoshitaka;Hosokawa, Ikuko;Matsuo, Takashi
CXC趋化因子配体10（CXCL 10）在Th 1细胞的浸润中起重要作用，从而在牙周病的恶化中起重要作用。红茶中的多酚茶黄素-3，3 '-二没食子酸酯（TFDG）具有一些有益作用，但TFDG对人牙龈成纤维细胞（HGF）产生CXCL 10的影响尚不确定。在这项研究中，我们研究了TFDG可能抑制抑瘤素M（OSM）诱导的人牙龈成纤维细胞CXCL 10的产生的机制。TFDG以剂量依赖性方式阻止OSM介导的HGF产生CXCL 10。TFDG显著抑制OSM诱导的c-Jun N末端激酶（JNK）、蛋白激酶B（Akt）（Ser 473）的磷酸化，这些激酶与OSM刺激的HGF产生CXCL 10有关。此外，TFDG抑制HGF上的OSM受体（OSMR）β表达。这些数据提供了一种新的机制，其中红茶黄酮，茶黄素，可以提供牙周病的直接好处。(C)2011 Elsevier B. V.保留所有权利。
CXC chemokine ligand 10 (CXCL10) plays an important role in the infiltration of Th1 cells and thus in the exacerbation of periodontal disease. Theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, has some beneficial effects but the effect of TFDG on CXCL10 production from human gingival fibroblasts (HGFs) is uncertain. In this study, we investigated the mechanisms by which TFDG may inhibit oncostatin M (OSM)-induced CXCL10 production in human gingival fibroblasts. TFDG prevented OSM-mediated CXCL10 production by HGFs in a dose dependent manner. TFDG significantly inhibited OSM-induced phosphorylation of c-Jun N terminal kinase (JNK), protein kinase B (Akt) (Ser473) that are related to CXCL10 production from OSM-stimulated HGFs. In addition, TFDG suppressed OSM receptor (OSMR) beta expression on HGFs. These data provide a novel mechanism where the black tea flavonoid, theaflavin, could provide direct benefits in periodontal disease. (C) 2011 Elsevier B.V. All rights reserved.