microRNA profiling between Bacillus thuringiensis Cry1Ab-susceptible and -resistant European corn borer, Ostrinia nubilalis (Hubner)
microRNA profiling between Bacillus thuringiensis Cry1Ab-susceptible and -resistant European corn borer, Ostrinia nubilalis (Hubner)
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苏云金芽孢杆菌 Cry1Ab 敏感和抗性欧洲玉米螟、Ostrinia nubilalis (Hubner) 之间的 microRNA 分析
DOI：
10.1111/imb.12376
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发表时间：
2018
影响因子：
2.6
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Yu T;Li X;Coates B S;Zhang Q;Siegfried B D;Zhou X
表达苏云金芽孢杆菌（Bt）晶体（Cry）蛋白毒素的转基因玉米杂交种有效地防止欧洲玉米螟（Ostrinia nubilalis），这是一种毁灭性的玉米害虫。田间监测和实验室选择检测到不同水平的家蚕对Cry1Ab毒素的抗性。microRNA（miRNAs）是参与转录后基因调控的短的非编码RNA。然而，它们在耐药性进化中的潜在作用在很大程度上仍然未知。对来自Cry1Ab敏感和抗性的欧洲玉米螟幼虫中肠的小RNA文库进行测序，发现了277个miRNAs，包括248个保守的和29个新的。实验室菌株之间的miRNA表达谱的比较分析预测，在Cry1Ab抗性幼虫的中肠中分别有26个和9个显著上调和下调的转录本。在通过定量真实的时间PCR检测的15个差异调节的miRNA中，9个（60%）被验证为在回交后代中与Cry1Ab抗性共分离。差异表达的miRNAs预计会影响参与细胞膜组分的转录物，这些转录物具有代谢和结合功能，以及推定的Bt抗性基因氨基肽酶Nan和钙粘蛋白。这些结果为进一步研究miRNAs在耐药性进化中的潜在作用奠定了基础。
Transgenic maize hybrids that express insecticidalBacillus thuringiensis(Bt) crystalline (Cry) protein toxins effectively protect against the European corn borer,Ostrinia nubilalis, a devastating maize pest. Field monitoring and laboratory selections have detected varying levels ofO. nubilalisresistance to Cry1Ab toxin. MicroRNAs (miRNAs) are short noncoding RNAs that are involved in post‐transcriptional gene regulation. Their potential roles in the evolution ofBtresistance, however, remain largely unknown. Sequencing of small RNA libraries from the midgut of Cry1Ab‐susceptible and resistantO. nubilalislarvae resulted in the discovery of 277 miRNAs, including 248 conserved and 29 novel. Comparative analyses of miRNA expression profiles between the laboratory strains predicted 26 and nine significantly up‐ and down‐regulated transcripts, respectively, in the midgut of Cry1Ab resistant larvae. Amongst 15 differentially regulated miRNAs examined by quantitative real‐time PCR, nine (60%) were validated as cosegregating with Cry1Ab resistance in a backcross progeny. Differentially expressed miRNAs were predicted to affect transcripts involved in cell membrane components with functions in metabolism and binding, and the putativeBt‐resistance genesaminopeptidase Nandcadherin. These results lay the foundation for future investigation of the potential role of miRNAs in the evolution ofBtresistance.