Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs.
Circular Concatemers of Ultra-Short DNA Segments Produce Regulatory RNAs.
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DOI：
10.1016/j.cell.2017.02.020
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发表时间：
2017-03-09
期刊：
影响因子：
64.5
通讯作者：
Nowacki M
中科院分区：
文献类型：
--
作者：
Allen SE;Hug I;Pabian S;Rzeszutek I;Hoehener C;Nowacki M
在纤毛原生动物草履虫tetraurelia中，Piwi相关的小RNA是在发育过程中消除数万个短的转座子衍生的DNA片段后产生的。然后，这些RNA在正反馈过程中靶向互补DNA以消除，从而有助于种系防御和基因组稳定性。在这项工作中，我们研究了这些RNA的形成，我们表明这些RNA是直接从短的（长度模式27 bp）切除的DNA片段转录的。我们的数据支持一种机制，即切除的DNA片段的连接和环化提供了RNA生产的模板。该过程允许产生用于Dicer样蛋白切割的双链RNA，以产生与切除的DNA序列精确匹配的小调控RNA群体。在草履虫中，缺失的DNA片段被转录形成调控RNA超短的DNA片段被连接并环化，从而允许转录这种连接是由连接酶IV进行的，它也修复DNA末端。连接是随机的，这导致了所产生的sRNA群体的多样性。
In the ciliated protozoan Paramecium tetraurelia, Piwi-associated small RNAs are generated upon the elimination of tens of thousands of short transposon-derived DNA segments as part of development. These RNAs then target complementary DNA for elimination in a positive feedback process, contributing to germline defense and genome stability. In this work, we investigate the formation of these RNAs, which we show to be transcribed directly from the short (length mode 27 bp) excised DNA segments. Our data support a mechanism whereby the concatenation and circularization of excised DNA segments provides a template for RNA production. This process allows the generation of a double-stranded RNA for Dicer-like protein cleavage to give rise to a population of small regulatory RNAs that precisely match the excised DNA sequences. In Paramecium, pieces of deleted DNA are transcribed to form regulatory RNAs Ultra-short DNA segments are concatenated and circularized, allowing transcription This concatenation is carried out by Ligase IV, which also repairs DNA ends Concatenation is random, which leads to diversity in the resulting sRNA population “Junk” DNA can be ligated into circles and transcribed to generate regulatory RNAs.