Novel anisotropic engineered cardiac tissues: Studies of electrical propagation
Novel anisotropic engineered cardiac tissues: Studies of electrical propagation
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DOI：
10.1016/j.bbrc.2007.07.138
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发表时间：
2007-10-05
影响因子：
3.1
通讯作者：
Tung, Leslie
中科院分区：
文献类型：
--
作者：
Bursac, Nenad;Loo, Yihua;Tung, Leslie
本研究的目的是设计具有均匀各向异性结构的心脏组织结构，并使用细胞膜电位的多位点光学作图来评估其电功能。将排列的蔗糖模板浸出制备的各向异性聚合物支架与新生大鼠心脏细胞接种，并在旋转生物反应器中培养6-14天。细胞在支架内排列并相互连接，当受到点电极刺激时，支持宏观上连续的各向异性脉冲传播。到培养第14天，沿心纤维传导速度与跨心纤维传导速度之比达到2，与天然新生儿心室相似，而动作电位持续时间和最大捕获率分别减少到120 ms和增加到5 Hz。较短的培养时间和较大的支架厚度与持续再入性心律失常的发生率增加有关。总之，这项研究是第一次成功尝试设计cm(2)大小的功能性各向异性心脏组织贴片。(C) 2007爱思唯尔公司版权所有。
The goal of this study was to engineer cardiac tissue constructs with uniformly anisotropic architecture, and to evaluate their electrical function using multi-site optical mapping of cell membrane potentials. Anisotropic polymer scaffolds made by leaching of aligned sucrose templates were seeded with neonatal rat cardiac cells and cultured in rotating bioreactors for 6-14 days. Cells aligned and interconnected inside the scaffolds and when stimulated by a point electrode, supported macroscopically continuous, anisotropic impulse propagation. By culture day 14, the ratio of conduction velocities along vs. across cardiac fibers reached a value of 2, similar to that in native neonatal ventricles, while action potential duration and maximum capture rate, respectively, decreased to 120 ms and increased to similar to 5 Hz. The shorter culture time and larger scaffold thickness were associated with increased incidence of sustained reentrant arrhythmias. In summary, this study is the first successful attempt to engineer a cm(2)-size, functional anisotropic cardiac tissue patch. (C) 2007 Elsevier Inc. All rights reserved.