Identification and characterization of novel and differentially expressed microRNAs in peripheral blood from healthy and mastitis Holstein cattle by deep sequencing
Identification and characterization of novel and differentially expressed microRNAs in peripheral blood from healthy and mastitis Holstein cattle by deep sequencing
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DOI：
10.1111/age.12096
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发表时间：
2014-02-01
期刊：
影响因子：
2.4
通讯作者：
Song, Ailong
中科院分区：
文献类型：
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作者：
Li, Zhixiong;Wang, Hongliang;Song, Ailong
MicroRNA(MiRNA)介导转录后基因调控，在调节免疫细胞的发育和调节包括牛在内的哺乳动物的先天免疫和获得性免疫反应中发挥重要作用。在本研究中，我们通过Solexa测序和生物信息学的方法，从健康荷斯坦牛和乳房炎荷斯坦牛的外周血中鉴定了新的和差异表达的miRNAs。总共检测到608个前体发夹(Pre-miRNAs)，编码753个成熟miRNAs。统计分析表明，有173个miRNAs(753个，22.98%)在健康奶牛和乳房炎奶牛之间有显著差异(P
MicroRNA (miRNA) mediates post-transcriptional gene regulation and plays an important role in regulating the development of immune cells and in modulating innate and adaptive immune responses in mammals, including cattle. In the present study, we identified novel and differentially expressed miRNAs in peripheral blood from healthy and mastitis Holstein cattle by Solexa sequencing and bioinformatics. In total, 608 precursor hairpins (pre-miRNAs) encoding for 753 mature miRNAs were detected. Statistically, 173 unique miRNAs (of 753, 22.98%) were identified that had significant differential expression between healthy and mastitis Holstein cattle (P