Characterization of Human Keratinocyte Cell Lines for Barrier Studies.
Characterization of Human Keratinocyte Cell Lines for Barrier Studies.
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用于屏障研究的人角质形成细胞系的表征。
DOI：
10.1016/j.xjidi.2021.100018
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发表时间：
2021-06
期刊：
影响因子：
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通讯作者：
Brewer MG
中科院分区：
文献类型：
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作者：
Moran MC;Pandya RP;Leffler KA;Yoshida T;Beck LA;Brewer MG
表皮细胞模型对于研究皮肤生物学至关重要。科学界使用的黄金标准历来是从废弃组织（通常来自新生儿（包皮））中进行原代细胞培养。虽然直接适用于人类，但该系统存在多个问题，包括供体间的巨大差异和培养中有限数量的分裂。因此，我们已经确定了一种称为N/TERT 2G细胞的可靠替代品。这些细胞显示出原代细胞的许多特征，包括屏障形成、分化动力学和/或蛋白质表达以及发病机制。根据我们的观察，N/TERT 2G细胞可以作为研究皮肤生物学的可重复和遗传操作的平台。
Epidermal cell models are critical for studying skin biology. The gold standard used by the scientific community has historically been primary cell cultures from discarded tissue, typically from neonates (foreskin). Although directly applicable to humans, this system suffers from multiple issues, including substantial donor-to-donor variability and a finite number of divisions in culture. As such, we have identified a faithful alternative called N/TERT2G cells. These cells show many of the characteristics of primary cells, including barrier formation, differentiation kinetics and/or protein expression, and pathogenesis. From our observations, N/TERT2G cells can serve as a reproducible and genetically manipulatable platform in studying skin biology.