In vitro cytotoxicity of nanoparticles in mammalian germline stem cells
In vitro cytotoxicity of nanoparticles in mammalian germline stem cells
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DOI：
10.1093/toxsci/kfi256
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发表时间：
2005-12-01
影响因子：
3.8
通讯作者：
Hofmann, MC
中科院分区：
文献类型：
--
作者：
Braydich-Stolle, L;Hussain, S;Hofmann, MC
配子发生是一个复杂的生物学过程，对诸如化学物质等环境侮辱特别敏感。许多化学物质通过直接影响生殖细胞或通过对躯体护理细胞的作用间接影响种系具有负面影响。最终，这些作用可以抑制生育能力，并且可能对后代的发展产生负面影响。最近，纳米材料（如纳米管，纳米线，富勒烯衍生物（Buckyballs）和量子点）在创建新型的生物技术和生命科学分析工具方面受到了极大的国家关注。尽管纳米材料广泛应用，但由于对它们对人类健康和环境的影响的严重缺乏信息。因此，关于纳米颗粒的毒性可用于纳米材料的风险评估的有限研究。这项研究的目的是评估小鼠精子干细胞系的适用性，作为评估体外男性种系的纳米毒性的模型。通过光学显微镜以及细胞增殖和标准的细胞毒性测定法评估了不同类型的纳米颗粒对这些细胞的影响。我们的结果表明，对所有测试的颗粒具有浓度依赖性毒性，而相应的可溶性盐没有显着影响。银纳米颗粒是最毒性的，而三氧化钼（MOO3）纳米颗粒的毒性最少。我们的结果表明，该细胞系提供了一个有价值的模型，可以通过该模型评估体外生殖系中纳米颗粒的细胞毒性。
Gametogenesis is a complex biological process that is particularly sensitive to environmental insults such as chemicals. Many chemicals have a negative impact on the germline, either by directly affecting the germ cells, or indirectly through their action on the somatic nursing cells. Ultimately, these effects can inhibit fertility, and they may have negative consequences for the development of the offspring. Recently, nanomaterials such as nanotubes, nanowires, fullerene derivatives (buckyballs), and quantum dots have received enormous national attention in the creation of new types of analytical tools for biotechnology and the life sciences. Despite the wide application of nanomaterials, there is a serious lack of information concerning their impact on human health and the environment. Thus, there are limited studies available on toxicity of nanoparticles for risk assessment of nanomaterials. The purpose of this study was to assess the suitability of a mouse spermatogonial stem cell line as a model to assess nanotoxicity in the male germline in vitro. The effects of different types of nanoparticles on these cells were evaluated by light microscopy, and by cell proliferation and standard cytotoxicity assays. Our results demonstrate a concentration-dependent toxicity for all types of particles tested, whereas the corresponding soluble salts had no significant effect. Silver nanoparticles were the most toxic while molybdenum trioxide (MoO3) nanoparticles were the least toxic. Our results suggest that this cell line provides a valuable model with which to assess the cytotoxicity of nanoparticles in the germ line in vitro.