Determination of Ten Organoarsenic Compounds Using Microbore High-performance Liquid Chromatography Coupled With Electrospray Mass Spectrometry–Mass Spectrometry
Determination of Ten Organoarsenic Compounds Using Microbore High-performance Liquid Chromatography Coupled With Electrospray Mass Spectrometry–Mass Spectrometry
复制标题
使用微孔高效液相色谱结合电喷雾质谱-质谱法测定十种有机砷化合物
DOI：
10.1039/a606416g
复制
发表时间：
1997
影响因子：
3.4
通讯作者：
D. Betowski
中科院分区：
文献类型：
--
作者：
S. Pergantis;W. Winnik;D. Betowski
反相微孔高效液相色谱联用分析方法
在线电喷雾质谱（ES-MS）。的
方法允许测定多达10种有机砷化合物，
单次色谱运行。出色的灵敏度和选择性，
通过操作三重四极杆质谱仪，
选择反应监测模式。这是可能的，因为离子
由质子化分子的碰撞诱导解离产生，或
十种有机砷化合物的分子离子是每种化合物的特征
化合物.在正离子模式下获得的最佳LOD在2
和21 pg砷，对应于以阳离子形式存在的砷
在酸性溶液中。通过使用该方法实现的选择性允许
用于成功测定HPLC期间共洗脱的砷。这是
这是对已经使用的其他连字符技术的重大改进，
砷形态，
例如
，HPLC-ICP-MS。此外，
方法用于分析未稀释的尿液SRM，其中
砷甜菜碱被确定为存在于低µg
L
-1
水平
An analytical method based on reversed-phase microbore HPLC coupled
on-line with electrospray mass spectrometry (ES-MS) is described. The
method allows for the determination of up to ten organoarsenicals in a
single chromatographic run. Excellent sensitivity and selectivity is
achieved by operating the triple quadrupole mass spectrometer in the
selected-reaction monitoring mode. This is possible because the ions
produced by collision-induced dissociation of the protonated molecules or
molecular ions of the ten organoarsenicals are characteristic of each
compound. The best LODs, achieved in the positive-ion mode, were between 2
and 21 pg of arsenic and corresponded to arsenicals which exist as cations
in acidic solutions. The selectivity achieved by using this method allows
for successful determination of arsenicals co-eluting during HPLC. This is
a major improvement over other hyphenated techniques already used for
arsenic speciation,
e.g.
, HPLC–ICP-MS. Furthermore, the
method was used for the analysis of an undiluted urine SRM in which
arsenobetaine was determined to be present at the low µg
l
-1
level.