Short-term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord
Short-term lineage analysis of dorsally derived Olig3 cells in the developing spinal cord
复制标题
DOI：
10.1002/dvdy.20545
复制
发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
Ono, K
中科院分区：
文献类型：
--
作者：
Ding, L;Takebayashi, H;Ono, K
我们检查了发育中脊髓中表达 Olig3（一种基本的螺旋-环-螺旋转录因子）的细胞的迁移和分化。通过原位杂交和 X-gal 染色在 Olig3-lacZ 敲入小鼠中证明了 Olig3 谱系细胞的分布。 Olig3阳性细胞首先出现在脊髓背侧（顶板除外）。一些背侧 Olig3 谱系细胞共表达 Islet1/2、Math1 或 Brn3a（背侧中间神经元的标记）。在 E10.5 脊髓的最腹侧部分观察到 LacZ 阳性细胞，表明一些背侧 Olig3 谱系细胞通过 E10.5 迁移到最腹侧部分。通过鸡胚背脊髓中 EGFP 表达质粒的电穿孔证实了背细胞的腹侧迁移和对连合中间神经元的贡献。背中线细胞在发育过程中也呈 LacZ 阳性。这些发现表明，背侧 Olig3 细胞有助于中间和腹侧水平的背侧中线细胞和连合中间神经元。
We examined the migration and differentiation of cells expressing Olig3, a basic helix-loop-helix transcriptional factor, in the developing spinal cord. Distribution of Olig3 lineage cells was demonstrated with in situ hybridization and X-gal staining in an Olig3-lacZ knock-in mouse. Olig3-positive cells first appeared in the dorsal spinal cord, except for the roof plate. Some of the dorsal Olig3 lineage cells co-expressed Islet1/2, Math1, or Brn3a, markers for dorsal interneuron. LacZ-positive cells were observed in the ventral-most part of the E10.5 spinal cord, suggesting that some dorsal Olig3 lineage cells migrate into the ventral-most part by E10.5. Ventral-ward migration of dorsal cells and contribution to commissural interneurons were substantiated by electroporation of EGFP expression plasmid in the dorsal spinal cord of chick embryo. Dorsal midline cells were also LacZ-positive during development. These findings suggest that dorsal Olig3 cells contribute to dorsal midline cells and commissural interneurons at intermediate and ventral levels.