Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.
Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.
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DOI：
10.1016/j.virol.2009.02.011
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发表时间：
2009-04
期刊：
影响因子：
3.7
通讯作者：
Cai-Hong Tian;Jin-fang Zhao;Yi-Peng Xu;J. Xue;Bao-Qin Zhang;Ying-Jun Cui;Min-juan Zhang;Yan-Yuan Bao;Chuan-Xi Zhang
中科院分区：
文献类型：
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作者：
Cai-Hong Tian;Jin-fang Zhao;Yi-Peng Xu;J. Xue;Bao-Qin Zhang;Ying-Jun Cui;Min-juan Zhang;Yan-Yuan Bao;Chuan-Xi Zhang
家蚕核型多角体病毒（BmNPV）ORF 41（Bm41）是存在于大多数鳞翅目核型多角体病毒中的基因，与Ac52同源。在BmNPV感染的细胞中，Bm41的转录本和编码的蛋白可以分别从感染后3和6 h检测到。免疫分析表明，Bm41不是一种病毒结构蛋白，在感染细胞的细胞核和细胞质中都能检测到。为了研究Bm41的功能，我们制备了Bm41破坏病毒（vBmDe）和修复病毒（vBmRe）。结果表明，Bm41是病毒复制所必需的，Bm41的破坏导致病毒滴度大大降低。透射电子显微镜显示，破坏Bm41影响正常的核衣壳的形成和多角体的形成在核中。Bm41的破坏可能严重影响odv-ec27和多角体蛋白的表达。在LD_（50）生物测定中，病毒的破坏降低了BmNPV的感染性，在LT_（50）生物测定中，病毒比野生型病毒多花18 - 23 h杀死幼虫。
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF41 (Bm41), homologous to Ac52, is a gene present in most lepidopteran nucleopolyhedroviruses. Bm41 transcripts and encoded protein in BmNPV-infected cells can be detected from 3 and 6 h post-infection, respectively. Immunoassays have shown that Bm41 is not a viral structural protein and is detected in both the nuclei and cytoplasm of infected cells. A Bm41-disrupted virus (vBmDe) and a repaired virus (vBmRe) were generated to investigate the function of Bm41. The results showed that Bm41 was essential for viral replication, and the disruption of Bm41 resulted in a much lower viral titer. Transmission electron microscopy revealed that disruption of Bm41 affected normal nucleocapsid envelopment and polyhedra formation in the nucleus. The disruption of Bm41 might severely affect odv-ec27 and polyhedrin expression. The disrupted virus reduced BmNPV infectivity in an LD50bioassay and took 18–23 h longer to kill larvae than wild-type virus in an LT50bioassay.