Co-existence of Paragonimus harinasutai and Paragonimus bangkokensis metacercariae in fresh water crab hosts in central Viet Nam with special emphasis on their close phylogenetic relationship
Co-existence of Paragonimus harinasutai and Paragonimus bangkokensis metacercariae in fresh water crab hosts in central Viet Nam with special emphasis on their close phylogenetic relationship
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DOI：
10.1016/j.parint.2012.01.011
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发表时间：
2012-09-01
影响因子：
1.9
通讯作者：
Nawa, Yukifumi
中科院分区：
文献类型：
--
作者：
Pham Ngoc Doanh;Hoang Van Hien;Nawa, Yukifumi
在越南中部地区对并殖吸虫种类的流行病学调查中，我们在山蟹体内发现了四种形态不同的并殖吸虫囊虫。经鉴定为卫氏并殖吸虫、棒状并殖吸虫、增殖并殖吸虫和哈氏并殖吸虫囊虫。这是在越南首次发现哈里纳苏泰肺吸虫，与邦戈巴克肺吸虫和其他物种共同生活。给猫口服哈氏并殖吸虫囊虫，以获得成虫。然后，对采集自同一地点的哈氏并殖吸虫囊虫和成虫以及邦科克肺吸虫囊虫的ITS2和CO1序列进行了测定，分析了它们与其他哈氏肺吸虫和邦科克肺吸虫种群及其近缘种的亲缘关系。分子分析结果表明，来自越南中部广平省的哈氏疟原虫与老挝人民民主共和国万象的同源性较高，与越南广平市的同源性较高，与老挝和越南广宁省的同源性较高。除中国分离的1株外，泰国、老挝和越南的哈氏并殖吸虫种群均在ITS_2和CO_1树上形成一个分支。在ITS2序列中，两个种的一些分离物中都存在AT缺失和ATC插入，这表明华支睾吸虫和邦戈氏假丝核菌之间最近出现了基因流动。此外，由于它们极高的遗传相似性，以及它们在泰国、老挝和越南发现的同一蟹宿主中共存，它们在分歧的早期阶段应被视为姊妹物种。此外，由于形态和分子上的相似性，原产于云南的微小拟青霉，其异名中国应归入青霉的异名。(C)2012爱思唯尔爱尔兰有限公司。保留所有权利。
During our epidemiological surveys for Paragonimus species in central Viet Nam, we found four morphologically different Paragonimus metacercariae in mountainous crabs. They were identified as metacercariae of Paragonimus westermani, P. bangkokensis, P. proliferus, and P. harinasutai in the order of their prevalence in crab hosts. This is the first discovery of P. harinasutai in Viet Nam, co-inhabiting with P. bangkokensis and other species. Metacercariae of P. harinasutai were given orally to a cat to obtain adult worms. Then, ITS2 and CO1 sequences of metacercariae and adults of P. harinasutai, and metacercariae of P. bangkokensis collected from the same place were determined for analyses of phylogenetic relationships to other P. harinasutai and P. bangkokensis populations as well as related species. The results of molecular analyses showed that P. harinasutai from Quang Binh province of central Viet Nam was almost completely identical with those from Vientiane, Lao PDR; P. bangkokensis from Quang Binh, Viet Nam was also almost completely identical with those from Lao PDR and from Quang Ninh province, Viet Nam. Except for one P. harinasutai isolate from China, all populations of P. harinasutai and P. bangkokensis from Thailand, Lao and Viet Nam make a single clade in both ITS2 and CO1 trees. In ITS2 sequences, AT deletion and ATC insertion were observed in some isolates of both species, indicating recent gene flow between P. harinasutai and P. bangkokensis. Moreover, because of their extremely high genetic similarities and their co-inhabitation in the same crab hosts found in Thailand, Lao PDR and Viet Nam, they should be considered as the sister species at the early stage of divergence. In addition, P. microrchis previously described from Yunnan, China should be placed as the synonym of P. harinasutai, because of their morphological and molecular similarities. (C) 2012 Elsevier Ireland Ltd. All rights reserved.